LipidFlux™ Sialic Acid Assay Kit

CAT#: ITS-1125-HM45
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies total sialic acid (N-acetylneuraminic acid) on glycoproteins.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Sialic Acid Assay Kit quantifies total sialic acid on glycoproteins, serving as a critical quality control tool for recombinant therapeutic proteins. This assay is also used to assess sialylation patterns, which are key markers of inflammation and tumor progression in diagnostic research. By validating the consistency of glycosylation, it ensures the efficacy and safety of biologic drugs.
Features
Detects both free and bound sialic acid; Supports quality control of recombinant therapeutic glycoproteins.
Process Relevance
Biologics Quality Control (Glycosylation)
Application Stage
Manufacturing / QC
Applications
Assessing glycoprotein sialylation in inflammation and cancer diagnostics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Inflammation; Bioprocessing (Glycosylation)
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 2 hrs
LOD
OD 20 µM; FL 2 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sialic acids are terminal monosaccharides on cell surface glycans. They mediate cell-cell interactions and pathogen binding and determining the stability and half-life of therapeutic proteins in circulation.
For Research Use Only | Not For Clinical Use
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