SigPath™ Human SHP2 (Y542) Phosphorylation ELISA Kit (CAT#: ITS-1125-WB5)

Quantifies phosphorylated SHP2 (Y542) levels in cell lysates using a sandwich ELISA method.

SigPath

Research-Grade Signal Pathway Assay Development

The SigPath™ Human SHP2 (Y542) Phosphorylation ELISA Kit offers a rapid, high-throughput solution for quantifying phosphorylated SHP2 in cultured cells without requiring lysate preparation. By normalizing phosphorylation levels to total protein content in the same well, it ensures high accuracy for screening drug candidates, siRNA, or pathway activators in signal transduction research.

Superior Analytical Sensitivity: Employs advanced chemiluminescent or colorimetric substrates to detect endogenous p-SHP2 levels in minimal sample volumes with high signal-to-noise ratios.
Validated Phospho-Specificity: Utilizes high-affinity capture/detection antibody pairs rigorously screened for zero cross-reactivity with non-phosphorylated SHP2 or related protein tyrosine phosphatases.
Streamlined High-Throughput Format: Optimized 96-well microplate architecture facilitates rapid screening of multiple experimental conditions or drug candidates in a single assay run.
Comprehensive Reagent Integration: Includes pre-coated plates, lysis buffers, and standardized controls to ensure maximal reproducibility and minimized intra-assay coefficient of variation.

Quantifying p-SHP2 (Y542) levels enables precise monitoring of phosphatase activation to evaluate kinase inhibitor efficacy and signal modulation.

Drug discovery, lead optimization, clinical biomarker validation, and longitudinal cell-based signaling studies.

Assessing SHP2 activation in lysates from mammalian cell lines, primary tissues, and xenograft models to study RAS/MAPK pathway dynamics and oncogenic transformation.

Internal performance validation using reference standards under defined assay conditions.

Human SHP2 protein specifically phosphorylated at the tyrosine 542 (Y542) regulatory residue.

Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 451 nm)

Cells

Oncology therapeutics, immunology and inflammation, growth factor signaling, cardiovascular biology, metabolic disorders, etc.