SigPath™ Human SHP2 (Y542) Phosphorylation ELISA Kit

CAT#: ITS-1125-WB5
Product Type: Assay Kit
Species: Human
Target: Human SHP2 protein specifically phosphorylated at the tyrosine 542 (Y542) regulatory residue.
Brand: SigPath
Development Stage: Research-Grade Signal Pathway Assay Development
Short Description
Quantifies phosphorylated SHP2 (Y542) levels in cell lysates using a sandwich ELISA method.
Brand
SigPath
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human SHP2 (Y542) Phosphorylation ELISA Kit offers a rapid, high-throughput solution for quantifying phosphorylated SHP2 in cultured cells without requiring lysate preparation. By normalizing phosphorylation levels to total protein content in the same well, it ensures high accuracy for screening drug candidates, siRNA, or pathway activators in signal transduction research.
Features
Superior Analytical Sensitivity: Employs advanced chemiluminescent or colorimetric substrates to detect endogenous p-SHP2 levels in minimal sample volumes with high signal-to-noise ratios.
Validated Phospho-Specificity: Utilizes high-affinity capture/detection antibody pairs rigorously screened for zero cross-reactivity with non-phosphorylated SHP2 or related protein tyrosine phosphatases.
Streamlined High-Throughput Format: Optimized 96-well microplate architecture facilitates rapid screening of multiple experimental conditions or drug candidates in a single assay run.
Comprehensive Reagent Integration: Includes pre-coated plates, lysis buffers, and standardized controls to ensure maximal reproducibility and minimized intra-assay coefficient of variation.
Process Relevance
Quantifying p-SHP2 (Y542) levels enables precise monitoring of phosphatase activation to evaluate kinase inhibitor efficacy and signal modulation.
Application Stage
Drug discovery, lead optimization, clinical biomarker validation, and longitudinal cell-based signaling studies.
Applications
Assessing SHP2 activation in lysates from mammalian cell lines, primary tissues, and xenograft models to study RAS/MAPK pathway dynamics and oncogenic transformation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human SHP2 protein specifically phosphorylated at the tyrosine 542 (Y542) regulatory residue.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 451 nm)
Sample Type
Cells
Research Areas
Oncology therapeutics, immunology and inflammation, growth factor signaling, cardiovascular biology, metabolic disorders, etc.
Size
100 tests
Data Output Type
Quantitative measurement of relative or absolute protein phosphorylation levels using endpoint absorbance.
Assay Time
6.5 h
Species
Human
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
SHP2, encoded by PTPN11, is a non-receptor protein tyrosine phosphatase essential for cellular proliferation and survival. Phosphorylation at Y542 relieves auto-inhibition, facilitating SHP2 interaction with Grb2 and subsequent RAS/ERK cascade activation.
Pathways
SHP2 acts downstream of most receptor tyrosine kinases (RTKs) and cytokine receptors. It functions as a critical relay in the RAS/MAPK, PI3K/Akt, and JAK/STAT signaling networks during cellular stimulation.
For Research Use Only | Not For Clinical Use
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