PharmaOnco™ SiHa Cancer Cell Line

CAT#: ITS-0523-LX81
Product Type: Cancer Cell Line
Organism: Human
Applications
in vitro and in vivo assay
Organism
Human
Tissue
Cervix
Disease
papillomavirus-related cervical squamous cell carcinoma
Morphology
Epithelial-like
Biosafety Level
BSL-2
Split Ratio
1:2 to 1:4
Medium Renewal
2 to 3 times per week
Oncology Cell Line
SiHa
Quantity
>1 million cell/vial
Unit Size
2 vials
Culture Properties
Adherent
Cell Viability
>80%
Sterility Testing
Sterility testing in accordance with USP and EP regulations.
Identity Testing
STR identification
Mycoplasma Testing
Negative
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank.
Warnings
Avoid multiple freeze/thaw cycles
COA

ITS-0523-LX81-1 Fig.1 Cell morphology of PharmaOnco™ SiHa Cancer Cell Line (CAT#: ITS-0523-LX81)

Cell Morphology

Cell morphology under the bright field.

ITS-0523-LX81-2 Fig.2 Mycoplasma Testing of PharmaOnco™ SiHa Cancer Cell Line (CAT#: ITS-0523-LX81)

Mycoplasma Testing

Mycoplasma contamination is detected by using a PCR Mycoplasma Test Kit. The kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The results showed that the cell line was negative for Mycoplasma.
Lane M: Marker;
Lane 1: Cell culture medium from SiHa;
Lane 2: Negative control;
Lane 3: Positive control;

For Research Use Only | Not For Clinical Use
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