Human microbiome analysis is the study of microbial communities found in and on the human body. With advanced next-generation sequencing and mass spectrometry platforms, Creative Biolabs provides comprehensive multi-omics services and bioinformatics analysis. We interpret complex and dynamic biological/clinical problems through more understandable readouts.
The field of metagenomics and other “omics” disciplines has greatly expanded due to improvements in sequencing technologies, namely “third-generation sequencing”, as well as analytical and single-cell techniques, allowing for a more comprehensive characterization of microbial communities and host-microbe interactions. Most microbiome studies employ “meta-omics” approaches, including 16S rRNA gene sequencing, metagenomics, metatranscriptomics, metaproteomics, and metabolomics, which directly examine the phylogenetic markers, genes, transcripts, proteins, or metabolites from the samples. Here are our omics platforms for studying gut microbiota.
Fig.1 Multi-omics integration approaches. (Lacroix, 2021)
Modern sequencing technology and computational analysis methodology are now widely used to associate changes in microbiome composition and function with human diseases. Generally, studying the molecular interactions using the combination of these omic approaches can considerably improve our understanding of gut microbiome. Multi-omics is an effective approach, as compared to studies of a single omics type, it offers the opportunity to understand the flow of information that underlies gut microbiota and host interaction. The commonest integrative multi-omic approaches that have been used over the years for microbiome studies are combinations of metagenomics and metatranscriptomics, metagenomics and metaproteomics, and metagenomics and metabolomics.
Microbiological cultures
Omics-based methods are now used as an alternative to traditional culture methods
16S rRNA gene amplicon sequencing
16s rRNA sequencing can be used to measure the composition of the gut microbiome.
Shotgun metagenomics sequencing
Shotgun metagenomics allows the identification of whole genomes including a larger range of micro-organisms (bacteria, fungi, viruses and protists).
Metabolomics
The determination of small-molecule metabolite profiles (metabolomes) can be performed using mass spectrometry or NMR spectroscopy.
Metatranscriptomics
The profiling of microbiome-wide gene expression can directly inform the activity of microbial communities.
Metaproteomics
Metaproteomics is the study of all protein samples, either using gel-based proteomics approaches or directly by shotgun proteomics.
As a world-leading biotechnology company, Creative Biolabs provide multi-omics technologies to solve complex problems in gut microbiology research. Our experienced scientists are happy to provide robust and comprehensive detection and analytical services, from sample preparation to statistical reporting. Please feel free to contact us for more details.
Reference
For Research Use Only | Not For Clinical Use
Download our brochure