Short Description
Quantifies phosphorylated AMPK (Thr172) in cell lysates using an ELISA-based method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ AMPK Phosphorylation Assay Kit provides a specialized cell-based solution for quantifying AMPK activation in response to metabolic stress and drug treatment. This high-throughput system eliminates cell lysis steps, enabling the rapid screening of energy metabolism modulators by normalizing phosphorylation levels to total protein content. By delivering precise, in situ data across diverse mammalian cell lines, it facilitates rigorous efficacy testing and mechanistic studies in diabetes and obesity research.
Features
Superior Sensitivity & Specificity: Employs advanced ELISA-based chemistry to detect endogenous Thr172 phosphorylated AMPK with high precision and minimal cross-reactivity.
Streamlined Homogeneous Format: Features a simplified "add-and-read" workflow that minimizes hands-on time and mitigates variability associated with multi-step processing.
Validated Matrix Compatibility: Optimized for robust performance across diverse biological systems, including mammalian cell lysates and complex tissue homogenates.
Process Relevance
The kit quantifies AMPK activation levels to facilitate the identification of metabolic regulators and the assessment of therapeutic potency.
Application Stage
Ideal for early-stage drug discovery, metabolic profiling, and high-throughput lead optimization in biopharmaceutical research and development.
Applications
Accurately assessing AMPK activation in cell-based assays, tissue samples, and pharmacological screenings to evaluate compounds influencing metabolic disorders, cancer, and age-related physiological decline.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
This kit specifically measures human, mouse, and rat AMPK alpha-subunit phosphorylation at Thr172.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 450 nm)
Research Areas
Metabolic syndromes, type 2 diabetes, oncology, cardiovascular disease, neurodegeneration, autophagy regulation, aging, etc.
Datasheet