Toll-like receptors (TLRs) are crucial in both innate and adaptive immune responses, making them key targets in drug development. At Creative Biolabs, we offer a comprehensive TLR family reporter panel screening service for screening potential therapeutic candidates, including therapeutic antibodies and proteins

Understanding TLR Family

TLRs are a subset of pattern recognition receptors essential for the innate immune response. They are expressed on various immune cells, including dendritic cells, macrophages, and B cells, acting as sentinels that detect pathogens and trigger immune responses. Upon activation, TLRs initiate signaling cascades that lead to the production of pro-inflammatory cytokines and promote the activation of adaptive immunity. There are 10 types of TLRs (TLR1-10) in human, each with a unique role in recognizing specific patterns associated with different microorganisms. With their ability to bridge innate and adaptive immune responses, TLRs are not only essential for pathogen recognition but also hold significant potential in therapeutic applications, including vaccine development and immunotherapy.

Fig.1 The TLR family and their localization in the cell.¹

Comprehensive TLR Reporter Panel Screening Service

At Creative Biolabs, we provide a robust family reporter panel screening service designed to facilitate the identification and characterization of novel therapeutic antibodies and proteins targeting TLRs. Our advanced screening platform leverages a set of highly sensitive and specific cell-based reporter assays tailored to various TLRs. Our advanced panels allow for the simultaneous screening of multiple TLRs, saving valuable time and resources while delivering comprehensive data sets. Our service provides invaluable insights into the efficacy of potential agonists or antagonists targeting specific TLR pathways. Whether you are working on therapeutic development, immunotherapy, or understanding infectious diseases, our services are designed to meet your needs and propel your research forward.

Our TLR Reporter Panels

Our TLR family reporter panel includes but not limited to TLR2, TLR4, TLR5, TLR7, TLR8, and TLR9. This assay facilitates the immediate tracking of TLR signaling in A-427 cells following stimulation with defined ligands.Our panels can be customized, providing clients with the flexibility to focus on particular TLRs relevant to their research. Our panels are optimized for high sensitivity and specificity, providing researchers with reliable and reproducible data essential for therapeutic development.

Applications

• Therapeutic Antibody and Protein Development
  Our TLR reporter panel supports the discovery and evaluation of antibodies and proteins that modulate TLR signaling. By screening for agonistic or antagonistic activity across multiple TLRs, researchers can identify candidates with immunomodulatory potential for treating autoimmune diseases, infections, and cancer.
• Vaccine Adjuvant Screening
  TLRs play a key role in vaccine-induced immune activation. Our platform allows for the evaluation of vaccine adjuvants by measuring their ability to activate specific TLR pathways, supporting rational adjuvant design and optimization of vaccine efficacy.
• Infectious Disease Research
  By simulating pathogen-associated molecular pattern (PAMP) recognition, our TLR screening assays help researchers study innate immune responses to various microbes. This aids in understanding host–pathogen interactions and identifying novel antimicrobial strategies.
• Immunotherapy Development
  The TLR reporter panel is an ideal tool for identifying compounds that can modulate the immune response in cancer or chronic inflammatory conditions. It enables rapid assessment of immune stimulators or suppressors that engage specific TLR pathways.

Collaboration with Industry Experts at Creative Biolabs

Creative Biolabs boasts a team of seasoned scientists with extensive expertise in immunology and related fields. Our experts offer a comprehensive range of TLR panels tailored to meet the diverse needs of researchers working in therapeutic development. For more information or to discuss how our TLR panel screening service can be tailored to your specific research needs, please contact us today.

Reference

1. Giurini, Eileena F., et al. "Microbial-derived toll-like receptor agonism in cancer treatment and progression." Cancers 14.12 (2022): 2923. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use