Glycan Analysis Service
O-linked and N-linked oligosaccharides covalently attached to proteins represent vital protein drug biomarkers. The microscopic heterogeneity of glycans is a key objective in vaccine development and drug discovery. Through the utilization of liquid chromatography and state-of-the-art mass spectrometry techniques, Creative Biolabs' focus lies in the precise identification of glycan structures and the determination of glycan patterns, providing invaluable insights into variations in glycan models or the identification of aberrant glycans.
Creative Biolabs Glycan Analysis Services
Armed with high-resolution and precision MS instruments, we offer our esteemed clientele an all-inclusive suite of services encompassing glycan profiling and structural analysis. Upon submission of your valuable test samples, we have developed efficient testing procedures adhering to stringent standard protocols. Our proficient team of bioinformatics specialists meticulously construct rational reports on glycan abundance and structure based on the comprehensive analysis of MS spectra.
Sample Collection
Submit your sample for testing, we have defined protocols for sample sources obtained in different formats (Antibody, protein, cell/tissue culture)
Glycan Release
Glycans will be released enzymatically or chemically, depending on the type of glycosylation.
Isolation
Released glycans are separated and desalted, and carefully inspected to remove any denaturants.
Derivatization
Glycan reducing end labeling or permethylation, ready for MS analysis.
MS Analysis
Glycan analysis by LC-MS or LC-MS/MS.
Data Analysis
Identifying glycans by fragmentation spectra.
Why Choose Us?
By choosing Creative Biolabs, you will benefit from:
Minimal sample requirements
Our cutting-edge MS tools possess heightened sensitivity, facilitating rapid generation of structural information even with limited sample quantities.
In-depth analysis
In addition to the elucidation and characterization of unknown compound structures, we provide comprehensive insights into branching patterns, substituent positions, and quantitative attributes.
Expedited responsiveness
We pledge prompt and timely delivery of the results, ensuring your research requirements are met without delay.
One-stop glycoprotein analysis
In addition to glycan analysis, we also complete glycosylation site detection and intact glycoprotein analysis.
Profound expertise
From sample processing to data analysis, our dedicated team of experts provides unwavering technical support, promptly addressing queries and resolving complex issues.
Our Services
With our state-of-the-art MS platform, Creative Biolabs enables high-throughput determination of glycan mass released from sample sources while minimizing sample consumption, facilitating an accurate assessment of structural information. We are also capable of providing other MS-based protein analyses and services.
Harnessing the specificity and sensitivity of tandem MS, we have the capability to measure a diverse array of biomolecules from limited samples across various sources, ultimately empowering your antibody research and biomarker discovery endeavors. Please feel free to contact us for consultations or to discuss your specific requirements.
Reference
- Han, L.; et al. Mass spectrometry of glycans. Biochemistry. 2013, 78(7): 710-720.