Peptide Mapping Service
Protein biopharmaceuticals exhibit significant heterogeneity during production, purification, handling, storage, and usage that affect the pharmacological characteristics, biological activity, pharmacokinetics, and safety of antibody drugs. By a combination of high-performance liquid chromatography (LC) and Mass Spectrometry (MS) platforms, Creative Biolabs can deeply characterize peptide mapping in biologics and protein products.
Peptide Mapping Identification Process
Creative Biolabs utilizes sequence-specific enzymes to digest protein samples, obtaining unique and predictable digestion fragments. These short peptides will be further separated through liquid chromatography to control the complexity of the submitted sample.
Obtained samples will be ionized and measured by MS for m/z. Our bioinformatics specialist will compare the experimental data systematically with peptide masses and fragment masses in the sequence database and provide you with the best-matched scoring results.
Sample Collection Protocol
We have developed different collection and digestion procedures for various types of protein samples. We welcome protein samples in the form of gel strips, gel blocks, or protein solutions. Any information regarding handling methods, buffer formats, and antibody types would be greatly appreciated.
Other sample formats (cell culture or tissue) are also acceptable. If you are unsure about the proper format or handling method for your sample submission, our team of experts is ready to provide technical guidance and support.
Fig 1. Peptide map and MS spectrum.1
What We Offer
Rapid and Thorough Protein Digestion
- Optimized workflows allow us to efficiently complete fixation, denaturation, reduction, alkylation, and digestion, seeking a balance between maximum digestion efficiency and minimal manual modification.
Powerful MS Analysis
- Cutting-edge LC-MS hybrid technology enables us to provide clear differentiation of isoforms and disulfide bond identification and localization of glycosylation.
Optimized Peptide Mapping Processing
- Standard sample handling procedures and data analysis programs ensure that variations in MS peak distribution are due to sample differences rather than human errors.
In-Depth Peptide Mapping Analysis
- Our professional and experienced bioinformatics engineers meticulously compare and calculate each entry in the experimental dataset and sequence database, providing precise localization of specific attributes.
Our Services
Peptide mapping has been widely used in the quality control and development of biological reagents and therapeutic mAbs. Creative Biolabs focuses on providing our clients with high-precision, high-resolution peptide mapping and target quantification. Our LC-MS hybrid technology platform allows for intuitive and in-depth analysis of almost any protein sample or antibody formulation, while other cutting-edge MS technology platforms are also available for your selection.
Our experienced team of scientists and technicians is equipped with state-of-the-art equipment to ensure high-quality and reliable results. Please feel free to contact us for consultations or to discuss specific requirements.
Reference
- Bhattacharya, S.; et al. A novel filter-assisted protein precipitation (FAPP) based sample pretreatment method for LC-MS peptide mapping for biosimilar characterization. Journal of Pharmaceutical and Biomedical Analysis. 2023, 10: 115527.