All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
The vector of anti-5T4 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target Human 5T4. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-5T4 antibody linked to 41BB and CD3ζ signaling domains. And the vector product was designed for the treatment of Nasopharyngeal carcinoma.
CAR Construction : Fig.1 FACS analysis of mAb and scFv 5T4 antibodies on A95T4a cells. mAb5T4 8 pM^16 nM, scFv5T4WT19 (470 pM^ 960 nM) and LscFv5T4 (360 pM^800 nM). 9E10 u-myc was used 1:10 as a control, rabbit u-mouse F(ab)2-FITC was used 1:40. Shaw, D. M., Embleton, M. J., Westwater, C., Ryan, M. G., Myers, K. A., Kingsman, S. M., Carroll, M. W., & Stern, P. L. (2000). Isolation of a high affinity scFv from a monoclonal antibody recognising the oncofoetal antigen 5T4. Biochimica et biophysica acta, 1524(2-3), 238–246. |
CAR Construction : Fig.2 Immunohistochemistry of normal term human placenta. scFv5T4WT19 or LscFv5T4 at 3 ug/ml. Shaw, D. M., Embleton, M. J., Westwater, C., Ryan, M. G., Myers, K. A., Kingsman, S. M., Carroll, M. W., & Stern, P. L. (2000). Isolation of a high affinity scFv from a monoclonal antibody recognising the oncofoetal antigen 5T4. Biochimica et biophysica acta, 1524(2-3), 238–246. |
CAR Construction : Fig.3 ELISA titration of 5T4 antibody variants. Monoclonal 5T4, scFv5T4WT19 and LscFv5T4 were titrated by doubling dilution against 5T4^IgGFc. Shaw, D. M., Embleton, M. J., Westwater, C., Ryan, M. G., Myers, K. A., Kingsman, S. M., Carroll, M. W., & Stern, P. L. (2000). Isolation of a high affinity scFv from a monoclonal antibody recognising the oncofoetal antigen 5T4. Biochimica et biophysica acta, 1524(2-3), 238–246. |
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