One-Stop CAR-NK Therapy Development

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

A new era of cancer immunotherapy has begun. Chimeric antigen receptor T (CAR-T) cells, which have been genetically engineered to recognize the CD-19 antigen of B cells, have been successfully treated in various clinical trials for relapsed and refractory B cell malignancies. However, this treatment has several disadvantages. Side effects such as cytokine toxicity, tumor lysis syndrome, effects on healthy tissues, B cell hypoplasia, and genotoxicity can be fatal.

To avoid these drawbacks, CAR technology is being applied to other immune cells, such as natural killer (NK) cells. Unlike T cells, NK cells can be more easily fine-tuned to prevent treatment-related toxicity and immune-mediated adverse events. NK cells have great clinical significance because they contribute to graft-vs-leukemia / graft-vs-tumor effect but are not responsible for graft-vs-host disease. NK cells can be generated from various sources, such as cord blood, bone marrow, human embryonic stem cells, and induced pluripotent stem cells.

CAR-NK vs CAR-T

Primary NK cells engineered to express CAR have potential advantages over CAR-T cells.

NK cells have spontaneous cytotoxic activity and can cause target cell death independent of tumor antigens, while T lymphocytes only kill their targets through CAR specific mechanisms. Therefore, NK cells will still be effective against tumor cells in the case of down-regulation of tumor cells that are trying to evade immune detection.
Primary human NK cells produce cytokines such as interferon gamma, interleukin 3, and granulocyte macrophage colony stimulating factor, which are different from the proinflammatory cytokines produced by T cells responsible for the onset of cytokine release syndrome.
A single NK cell can survive after contacting and killing multiple target cells, which may reduce the number of cells that require adoptive transfer, as opposed to T cells.
In addition, although the long-term persistence of CAR-T cells can maintain on-target and off-tumor toxicity, mature NK cells have a short lifespan and are expected to disappear after promoting their anti-cancer effects.

Therefore, NK cell lines are attractive for CAR cell therapy.

As the leading cell therapeutics biotech that provides cell therapy related services, Creative Biolabs masters the most advanced CAR technology. With state-of-art CAR development platforms and advanced technologies, Creative Biolabs is capable of offering a broad range of CAR-NK early development services, including CAR engineered T cell biomarker identification and selection, design, construction, and analysis.

Biomarker Identification & Selection

De novo discovery of potential cancer biomarkers by multiple gene and protein analysis technologies
Selection of the most suitable CAR-NK therapy targets with greater potency and lower toxicity

High Affinity ScFv Generation

In-house inescapable antibody sequence data base
A collection of over 6000 antibody products
Antibody discovery by hybridomas, mouse, rabbit and human library
ScFv sequencing and NGS of hybridomas or phage libary
ScFv affinity measurement and optimization

CAR-NK Vector Design and Construction

Design and construction of CARs of different generation
Smart™ CAR platform for the next generation CAR discovery
Customized lentiviral or retroviral CAR construction

Virus Packaging & CAR Gene Delivery

High titer transduction-ready pseudoviral particles produced by our cell manufacture platform
Strict quality control
Various transfection technology available
Transfection method tailored for different cell types

CAR-NK In Vitro Assay

CAR expression validation
CAR-NK cell proliferation
Multiplex cytokine screening
Cytotoxicity test against target cells

CAR-NK Preclinical In Vivo Assay

Construction of Xenograft Animal Model
Efficacy Test of CAR-NK cells
Viability and Bio-distribution Study of CAR-NK cells
Toxicity Evaluation of CAR-NK cells
GLP-compliant assays

CAR-T Clinical Trial

Study design and filing
Project management and execution
Data management and biostatistics
QA&QC

Creative Biolabs has also developed a series of novel platforms for enhanced CAR-NK Therapy. Please visit: