As a leading custom service provider in the field of T cell receptor (TCR) development, Creative Biolabs has highly qualified groups, advanced technologies and wide platforms to perform the most comprehensive TCR analysis, and our first-class TCR assay system covers the assessment of TCR clonality, the analysis of CDR3 diversity as well as the TCR Vα/β repertoire evaluation. We are committed to providing our customers accurate assay data and help every customer develop better TCR products in a time-saving and cost effective way.
TCR is expressed on the surface of T cell and recognizes diverse antigens such as proteins, polysaccharides, bacteria or viruses. Usually, the TCR gene includes an α-chain and a β-chain, the former consists of variable (V), joining (J) and constant (C) gene fragments, while the TCR β-chain is made up of a V, J and C region, with an additional diversity (D) region and these genes can be grouped into a distinct TCR Vβ gene family. What’s more, the rearrangement of those TCR genes plays a crucial role in antigen recognition by T cells, which enables T cells to recognize antigens specifically, and any dysregulation in this complex yet highly regulated process may lead to disease. Therefore, in T-cell immunotherapy development, the analysis of TCR repertoire clonality and diversity can serve as an important approach for evaluating virus, tumor or self-immune antigens-induced T lymphocytes clone types. Moreover, current evidence demonstrates that fast off-rates and serial engagement of MHC-peptide and TCR complexes may be crucial for T-cell activation, and tetramerized MHC class I and class II complexes have been used extensively to quantify and characterize antigen-specific T cells and to probe TCR-MHC interaction. The accessibility of various MHC-peptide tetramers can be great helpful for the study of specific TCR. With full understanding of the function and working mechanism of TCR, scientists of Creative Biolabs carry out custom TCR analysis services which include TCR-repertoire sequencing, TCR Vβ Repertoire Analysis, MHC-peptide tetramer, TCR clonality assessment and Clonality quantitation and CDR3 size diversity determination. Meanwhile, our scientists have developed a novel assay technique to overcome the shortcoming of traditional techniques (e.g. flow cytometry and PCR) in TCR clonality assessment. We guarantee that reliable, rapid, sensitive and quantitative analysis will be performed throughout your whole TCR assay project. For each TCR analysis project, Creative Biolabs will present and deliver a comprehensive report including detailed procedures and results for your project, such as analytical methods, all analysis results, appropriate graphs and calculations. All raw data is also available upon request.
Based on our combinatory and comprehensive package of analytical methods, Creative Biolabs has come to the unique position to not only have the technology and experience necessary for all kinds of TCR analysis, but also the capability to provide these analytical services in the most cost and time efficient manner.
Laydon et al., 2015. Estimating T-cell repertoire diversity: limitations of classical estimators and a new approach. Philos Trans R Soc Lond B Biol Sci. 19;370(1675).
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