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Anti-CD56 (T199)-CD147 CAR THP1 (CARTHP-W2179)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-CD56 chimeric antigen receptor (CAR) THP1 cell line is a stable cell line developed using anti-CD56 CAR lentivirus transfection. This transfected CAR lentiviral vector is constructed to express a scfv of anti-CD56 antibody (T199) linked to CD147 signaling domains.The anti-CD56 CAR engineered THP1 cells can be used to be differentiated into macrophage cells for in vitro or in vivo phagocytosis studies. This CAR-THP1 cell line product can be used for the researches of Neuroblastoma.

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  • Marker:
  • Positive rate:
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Details

  • Target
  • CD56
  • Cell Type
  • THP1
  • Cell Background
  • THP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug transport. This cell line has become a common model to estimate modulation of monocyte and macrophage activities. CAR engineered THP1 cells can be differentiated into macrophages using PMA (Phorbol 12-myristate 13-acetate). Once differentiated (M0 macrophages), they were incubated with IL-4 and IL-13 in order to obtain M2 polarized macrophages or with IFN-gamma and LPS for classical macrophage activation (M1).
  • Targeting Diseases
  • Neuroblastoma
  • CAR Expression
  • Positive
  • CAR Positive Rate
  • >95% for stable cell pool (please submit an inquiry if single clonal cell line is needed.)
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • Negative
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Safety Consideration
  • Biosafety Level 1
  • Restriction
  • Research use only
  • Size
  • 2 million cells*2 vials
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • T199
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • CD56
  • Target Species
  • Human
  • Clone
  • T199
  • Host
  • Mouse
  • Gene Name
  • Neural cell adhesion molecule 1
  • Introduction
  • This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
  • Alternative Names
  • NCAM1; neural cell adhesion molecule 1; CD56; NCAM; MSK39

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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