Anti-SLAMF7 (E5C4M) h(4-1BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
SLAMF7

Targeting Cell Type
T cell

Targeting Diseases
Lung malignancy

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-4-1BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigennonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

Customize Your CAR Products

Published Data

Complete CAR data ELISA

Fig.1 Determination of cytokines release.

CAR Construction : luc90-CD28-CD3ζ, luc90-41BB-CD3ζ

Fig.1 Determination of cytokines release.

Compared with T cells expressing Luc90-CD8BBZ, T cells expressing Luc90-CD828Z released higher levels of IFN-γ, IL-2, and tumor necrosis factor (TNF)-α when stimulated with MM.1S target cells in vitro.

Amatya, C., Pegues, M. A., Lam, N., Vanasse, D., Geldres, C., Choi, S., ... & Kochenderfer, J. N. (2021). Development of CAR T cells expressing a suicide gene plus a chimeric antigen receptor targeting signaling lymphocytic-activation molecule F7. Molecular Therapy, 29(2), 702-717.

Complete CAR data BI

Fig.2 Anti-tumor Activity of luc90-CAR-T cells.

CAR Construction : luc90-CD28-CD3ζ, luc90-41BB-CD3ζ

Fig.2 Anti-tumor Activity of luc90-CAR-T cells.

Anti-tumor efficacy of Luc90-CD8BBZ and Luc90-CD828Z CAR T cells was evaluated in NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice bearing solid tumors of the MM.1S human MM cell line.

Complete CAR data Cyt

Fig.5 In vitro cytotoxicity of luc90-CAR-T cells.

CAR Construction : luc90-CD28-41BB-CD3ζ

Fig.5 In vitro cytotoxicity of luc90-CAR-T cells.

Luc90-CAR-T and CD19-CAR-T effectors were incubated with 51chromium labeled MM.1S-CG or MM.1S-ΔCS1 target cells for 4 h. Chromium release was used to assess killing.

O’Neal, J., Ritchey, J. K., Cooper, M. L., Niswonger, J., Sofía González, L., Street, E., ... & DiPersio, J. F. (2022). CS1 CAR-T targeting the distal domain of CS1 (SLAMF7) shows efficacy in high tumor burden myeloma model despite fratricide of CD8+ CS1 expressing CAR-T cells. Leukemia, 36(6), 1625-1634.

Complete CAR data BI

Fig.6 Anti-tumor Activity of luc90-CAR-T cells.

CAR Construction : luc90-CD28-41BB-CD3ζ

Fig.6 Anti-tumor Activity of luc90-CAR-T cells.

Anti-tumor efficacy of Luc90-CAR-T was evaluated in NGS mice injected with MM.1S-CG cells.

Complete CAR data BI

Fig.7 Anti-tumor Activity of CAR-T cells.

CAR Construction : luc90-CD28-41BB-CD3ζ, luc90-41BB-CD3ζ

Fig.7 Anti-tumor Activity of CAR-T cells.

Anti-tumor efficacy of the third generation Luc90-CAR-T, Luc90-BBz-CAR-T were compared with MND-Luc90-BBz CAR-T (lower CAR copy number) in MM.1S-CG mouse model.

More Published Data More Published Data

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-SLAMF7 (E5C4M) h(4-1BB-CD3ζ) CAR, pCDCAR1 (CAR-ZP7307)

Specific Inquiry

Details

Target

Customize Your CAR Products

Customer Reviews and Q&As

Related Products

CAR Vector Products

CAR Cell Products

CAR mRNA

CAR Animal Cells

Protein