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pCDCAR1 CD20 h(BBζ) (CAR-YF820)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-CD20 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human CD20. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-CD20 antibody linked to 41BB and CD3ζ signaling domains. And the vector product was designed for the treatment of NHL.

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Details

  • Target
  • CD20
  • Targeting Cell Type
  • T Cell
  • Targeting Diseases
  • NHL
  • Generation
  • Second
  • Vector Name
  • pCDCAR1
  • Vector Length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Receptor Construction
  • scFv-41BB-CD3ζ
  • Discription of Signaling Cassetes
  • 41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.

    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Clone
  • 2B8
  • Host
  • Mouse
  • Target Species
  • Human
  • Gene Name
  • membrane spanning 4-domains A1
  • Synonyms
  • B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; BA0185

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  • Published Data
CAR scFv data ELISA

Fig.1 Analysis of antibody binding to CD20 + CHO by whole cell ELISA.

CAR Construction : Latest CAR Construction

Fig.1 Analysis of antibody binding to CD20 + CHO by whole cell ELISA.

Antibody reactivity to CD20 + CHO fixed on a PLL plate was tested.

Nishida, M., Usuda, S., Okabe, M., Miyakoda, H., Komatsu, M., Hanaoka, H., ... & Niwa, O. (2007). Characterization of novel murine anti-CD20 monoclonal antibodies and their comparison to 2B8 and c2B8 (rituximab). International journal of oncology, 31(1), 29-40.

CAR scFv data FCM

Fig.2 The anti-CD20 binding activity of CD20-binding polypeptide compositions and immunocytokines.

CAR Construction : Latest CAR Construction

Fig.2 The anti-CD20 binding activity of CD20-binding polypeptide compositions and immunocytokines.

Human Daudi lymphoma cells were incubated with varying concentrations of polypeptide compositions and ICs and relative binding to the cells was assessed by flow cytometry.

Carr, F. J., Williams, S., & Gillies, S. D. (2012). U.S. Patent No. 8,147,832. Washington, DC: U.S. Patent and Trademark Office.

More Published Data More Published Data

Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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