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TET2 Knockout Anti-hCD20 (28BBζ) CAR-Jurkat Cell Line (XS-0124-LX671)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Anti-CD20 (28BBζ) CAR-Jurkat Cell Line with TET2 knockout conducting epigenetic reprogramming strategies to accelerate CART project.

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Specifications

  • Species
  • Human
  • Host Cell Type
  • T lymphocyte
  • Size
  • 2 million cells per vial
  • Culture Condition
  • Suspension
  • Growth Pattern
  • Exponential growth phase
  • Formulation
  • Frozen cells
  • Cell Purity
  • >90%
  • Cell Viability
  • >80%
  • Mycoplasma Testing
  • Negative
  • Shipping
  • Dry ice
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank.
  • Warnings
  • Avoid multiple freeze/thaw cycles.
  • Research Use Only
  • RUO
  • Quality Control
  • Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
  • Application
  • Proof of concept studies for CAR-T research.

CAR Design

  • Target
  • CD20
  • Target Species
  • Human
  • scFv Clone
  • GA101
  • scFv Host Spcies
  • Mouse
  • CAR Construction
  • h(CD28-4-1BB-CD3ζ)
  • CAR Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    41BB
    CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ, ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric ICOS and 4-1BB can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
  • CAR Vector Name
  • pCDCAR1
  • CAR Vector length
  • ~8kb
  • CAR Vector Type
  • Lentiviral vector
  • CAR Generation
  • Third
  • Targeting Diseases
  • Acute lymphoblastic leukemia (ALL)

KO Targets

  • KO Targets
  • TET2
  • KO Targets Full Name
  • Methylcytosine dioxygenase TET2
  • Applcation of KO Targets
  • TET2 exerts important function on chromatin modification in cells.
  • Strategies Type
  • Epigenetic Reprogramming Strategies
  • Reference
  • Fraietta, Joseph A., et al. "Disruption of TET2 promotes the therapeutic efficacy of CD19-targeted T cells." Nature 558.7709 (2018): 307-312.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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