Creative Biolabs has developed several effective strategies to assess the performance of gene circuits in engineered cancer cells, including quantification of circuit-induced cell death, fluorescent reporter assays, and apoptosis assays for the measurement of specificity and selectivity. Our years of experience, deep resources, and flexible service options will help deliver reliable support for any phase of your project.
Precision medicine in oncology needs to enhance its capabilities to match therapeutic technologies to individual patients. Synthetic biology is primed to provide the conceptual framework and genetic tools necessary to enhance cell-based therapeutics. Immunotherapy has shown great promise for eradicating tumors in clinical trials. Remarkable improvements have opened the prospects for the availability of synthetic devices with enhanced mechanism clarity, robustness, sensitivity, as well as scalability and portability. Much of the success in therapies based on engineering T cell receptors (TCRs) and chimeric antigen receptors (CARs) has been achieved. Nowadays, synthetic circuits are primed to improve T cell therapy efficacy and meet safety concerns, such as discriminative capacity between tumors and vital organs and potential adverse side effects. This might bring new capabilities in precision cancer medicine implementations.
Fig.1 Fluorescent reporter assays and killing experiments in cell mixtures. (Xie, et al., 2011 )
Engineered cancer cells that integrate multi-input sensing, sophisticated information processing, and precisely regulated actuation in cells could be useful in the treatment of cancer. Creative Biolabs has established a cutting-edge platform to Engineer Cancer Cells With Synthetic Gene Circuits, turning cancer cells against themselves. There are several methods to assess the performance of the synthetic gene circuits, as follows.
Quantification of circuit-induced cell death is one of the methods for circuit performance assessment. At Creative Biolabs, a plethora of methods for studying cell death are now available, including membrane permeability/damage detection methods, mitochondrial damage/alteration detection methods, caspase activity detection methods, p53 activity detection methods, and DNA -based detection methods.
An important measure of circuit performance is specificity and selectivity in heterogeneous cell populations. Cocultured fluorescent HEK293-Cerulean cells and unmodified HeLa cells can be used for this purpose.
A large number of methods are devoted to the identification of apoptotic cells. Many methods have been developed to analyze the morphological, biochemical, and molecular changes that take place during this universal biological process. Creative Biolabs has developed an extensive suite of approaches to measure various forms of cell death, including flow cytometry, Trypan Blue, Hoechst staining, and annexin V-fluorochrome conjugates. We provide highly sensitive & reproducible assays for the detection of apoptosis induced by the circuit. Key biomarkers, such as caspase activity, DNA fragmentation, and translocation of phosphatidylserine to the out surface of the plasma membrane, can be efficiently detected.
For more detailed information, please feel free to contact us or directly send us an inquiry.
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