Based on a gut microbiota analysis platform, Creative Biolabs offers one-stop services to determine the relative abundance of Lactobacillus reuteri and HDC genes in vivo. We are dedicated to providing the highest level of services in gut microbiota research.
The human intestine contains thousands of bacterial species essential for optimal health. The gut microbiota has been reported to play key roles in the regulation of several processes related to brain function and mental health. Gut microbes may communicate with the gut-brain axis via the production of neuroactive and neuroendocrine molecules such as serotonin, GABA, histamine, noradrenaline, and adrenaline.tract.
L. reuteri is a well-studied probiotic bacterium that can colonize a large number of mammals. Lactobacillus generally are the resident microbiota in the human gastrointestinal tract. L. reuteri strains play an important role in antimicrobial and immunomodulatory effects by their metabolite production profile, such as organic acids, ethanol, reuterin, histamine and vitamins.
Fig.1 Probiotic properties of L. reuteri. (Mu, 2018)
Relative abundances of L. Reuteri can be determined by fluorescence in situ hybridization (FISH) and qPCR. In the FISH method, biospecimens such as colons are fixed in Carnoy's solution. The fluorescent probes are nucleic acid with fluorescent groups and can bind to specific DNA/RNA sequences in L. reuteri. The hybridized probes are detected and visualized using fluorochrome-conjugated reagents by fluorescence microscopy. The relative abundance of L. reuteri is determined by qPCR using specific primers and universal primers as normalizers of the abundance of L. reuteri. In addition, high-throughput sequencing (HTS) of 16S rRNA gene amplicon is a reliable and widely used method used to describe the bacterial relative abundance.
Human-derived clade II L. reuteri strains contained a complete chromosomal HDC gene cluster and the genetic capacity to convert histidine to histamine. We isolate bacterial DNA and RNA from biospecimens and evaluate relative bacterial DNA and mRNA quantities of HDC genes by q-PCR.
Creative Biolabs is a biotechnology company that solves complex problems in gut microbiology research. We have rich experience in FISH and qPCR arrays, and provide extensive microbial detection probes and corresponding in situ hybridization services. We also have established an accurate, sensitive, and high-throughput technologies platform for gut microbiota multi-omics analysis. Our experienced scientists will offer the most suitable strategies according to your sample and research purpose. If you have any questions or specific requirements, please don’t hesitate to contact us.
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