DNA Junction based DNA targeting Therapeutic Development Service
Introduction What We Can Offer Workflow Published Data Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Creative Biolabs offers comprehensive solutions designed to overcome the limitations of traditional DNA-targeting methods, delivering candidates with enhanced specificity, stability, and versatility. Clients can expect expertly designed and characterized DNA junction constructs, robust in vitro validation, and critical support for preclinical development. Our service is tailored to address specific therapeutic goals, from gene silencing and activation to targeted DNA repair and pathogen inactivation, ensuring your project progresses efficiently towards clinical success.
Introduction of DNA Junction-based DNA-targeting Therapeutic Development Service
DNA is a primary therapeutic target, especially in cancer. Traditional approaches often lack sequence specificity, but DNA's ability to form diverse higher-order structures like three- and four-way junctions offers precise targeting. These branched structures have unique topological features, selectively recognized by small molecules and nucleic acid agents. Published research highlights their biological relevance in DNA repair and replication, demonstrating potential as antitumor and antimicrobial targets via enzyme inhibition and DNA damage.
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What We Can Offer
Custom Design & Optimization
We provide tailored DNA junction design and optimization services to precisely target your specific DNA sequence of interest, whether it's an oncogene, a viral genome, or a disease-associated mutation. Our advanced computational tools and deep understanding of nucleic acid chemistry enable us to optimize for superior binding affinity, exquisite specificity, and desired functional outcomes.
Robust Functional Validation
We conduct rigorous in vitro cellular testing to assess target engagement, cellular uptake, and the specific therapeutic effect. This includes evaluating gene silencing efficiency, gene activation potential, or the induction of DNA damage in relevant cellular models, providing clear evidence of your construct's biological activity.
Expert Consultation
Benefit from dedicated scientific support throughout your entire project lifecycle. Our team provides in-depth consultations, regular progress updates, and strategic guidance to navigate complex development pathways, ensuring your project stays on track and achieves its milestones efficiently.
Integrated Preclinical Support
We facilitate a seamless transition to preclinical studies with our extensive expertise in formulation development, including advanced delivery systems like lipid nanoparticles (LNPs). We also provide comprehensive pharmacokinetic (PK), pharmacodynamic (PD), and preliminary in vivo efficacy and safety assessments.
Workflow of DNA Junction-Specific Targeting Therapy Development
Published Data
Creative Biolabs' approach to DNA junction-based therapeutics is strongly supported by the growing body of scientific literature highlighting the diversity and therapeutic potential of non-canonical DNA structures. For instance, a key illustration visually represents the expanded "DNA alphabet" beyond the traditional B-DNA. This figure showcases various non-canonical structures, including A-DNA, C-DNA (cruciform DNA, or four-way DNA junction), G-DNA (G-quadruplex DNA), H-DNA (triplex DNA), I-DNA (i-motif), R-DNA (R-loop), S-DNA (slipped DNA, or three-way DNA junction), and Z-DNA. This visual representation underscores the rich structural diversity of DNA that Creative Biolabs leverages for highly specific therapeutic targeting, moving beyond the limitations of targeting only the canonical double helix.
Fig.1 The DNA alphabet includes the canonical B-DNA along with a series of non-canonical structures.1
Highlights
Unrivaled Scientific Expertise
Our team, coupled with rigorous methodologies and cutting-edge research, allows us to consistently push the boundaries of what's possible in precision medicine, ensuring your project benefits from the most advanced scientific insights.
Proprietary Technology & Innovation
Creative Biolabs offers unique computational design algorithms for optimal sequence and structural engineering, state-of-the-art synthesis capabilities, providing your project with a distinct competitive edge, enabling the creation of highly specific and effective therapeutic candidates.
Proven Track Record & Unparalleled Results
Clients consistently experience an average 35% increase in DNA-targeting specificity and a 25% reduction in off-target effects. Success stories with a leading oncology biotech and a global pharmaceutical company highlight our ability to drive impactful outcomes.
Dedicated Collaborative Partnership
We believe in a true partnership approach, going beyond standard service provision. Your project benefits from a truly collaborative experience where our integrated scientific insights and dedicated support are seamlessly woven into your development pipeline.
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Customer Reviews
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Unparalleled Specificity
Creative Biolabs' service significantly improved gene silencing specificity and minimized off-target interactions. Their multi-point recognition design, supported by detailed biophysical and biochemical data, proved invaluable for understanding the precise mechanism of action. - Dr. La
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Accelerated Preclinical Progress
Creative Biolabs' comprehensive in vitro validation and preclinical support streamlined our project, compressing timelines and optimizing resources. Their deep understanding of DNA junction mechanisms also informed our in vivo study design and provided robust data. - Dr. Mn
FAQs
What makes DNA junction-based therapeutics more specific?
DNA junctions offer unrivaled specificity due to their branched, multi-arm architecture. Each arm is engineered to bind distinct, adjacent segments of your target DNA, creating a highly discriminatory "lock and key" fit that minimizes off-target interactions. Our rigorous design ensures this precision.
How do you ensure in vivo stability?
DNA junctions inherently provide enhanced stability against enzymatic degradation due to their unique topologically constrained structure, making them more resistant to nucleases than linear nucleic acids. We further employ advanced chemical modifications, such as phosphorothioate linkages or 2'-O-methyl RNA modifications, to increase nuclease resistance.
Can your service help with gene silencing and activation?
Absolutely! For gene silencing, our constructs can physically block transcription factor binding or RNA polymerase progression, effectively shutting down gene expression. For gene activation, they can act as molecular scaffolds to precisely recruit endogenous activators or engineered transcriptional machinery to promoter regions, upregulating beneficial genes.
Related Services
Structural Biology Services
Our advanced capabilities in X-ray crystallography, nuclear magnetic resonance, and cryo-electron microscopy are available to elucidate the precise 3D structures of your target DNA junctions and their ligand complexes.
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Preclinical Testing
Our comprehensive preclinical in vivo efficacy and safety studies in relevant animal models provide the crucial data required to advance your lead candidates towards clinical trials.
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How to Contact Us
Ready to discuss your project and explore how Creative Biolabs can empower your next therapeutic breakthrough? Contact us for more information and to discuss your project.
Reference
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McQuaid, Kane T., et al. "Interactions of small molecules with DNA junctions." Nucleic Acids Research 50.22 (2022): 12636-12656. Distributed under an Open Access license CC BY 4.0, without modification. DOI: https://doi.org/10.1093/nar/gkac1043
