Creative Biolabs offers tumor lineage tracing, aiming to provide reliable, reproducible, and manageable research tools for oncology imaging. This Dre-rox recombination platform is designed to accelerate the research and development of novel genetic lineage tracing approaches. We are committed to bringing a better simulation of the actual situation in vivo tumor lineage tracing solutions and providing customers with more real and accurate imaging information and data.

Introduction to Dre-rox Recombination System

Dre (D6 site-specific DNA recombinase) is a tyrosine recombinase discovered from a bacteriophage that recognizes a 32bp DNA sequence rox consisting of two 14bp reverse palindromic sequences and an intermediate spacer sequence of 4bp. Dre recombinases can only specifically recognize the rox site but not the other sites.

In recent years, the Dre-rox site-specific recombination system has proven its significant role in DNA and genome engineering. It has been regarded as one of the most valuable alternative systems for Cre-loxp. Therefore, we have developed a Dre-rox system based on a variety of Dre expression constructs. In general, a mammalian codon-optimized CBL plasmid was cloned into the pCBH001-Dre in Escherichia coli, resulting in complete recombination between rox sites. Our Dre-rox recombination system can effectively avoid the potential non-specific homologous recombination issue and can be used for accurate genetic lineage tracing, laying a reliable technical foundation for further research in the field of tumor occurrence, development, and diagnosis.

Services

To date, we have built a variety of animal models of genetic tools based on the Dre-rox recombinant enzyme system for global customers and designed a variety of new strategies for tumor lineage tracing and targeting, which not only achieve accurate analysis but also greatly broaden the scope of application. Our services include, but are not limited to:

• Gene knockout animal construction services
  In our labs, our staff can provide genetically modified animal-dependent gene knockout services. A more common form of genetic engineering in Creative Biolabs using the dre-rox system is the hybridization of two transgenic animals.
• Virus-dependent gene knockout services
  Due to the many drawbacks of transgenic animals, we chose a new way to construct conditional gene knockout mice, namely the virus-dependent dre-rox recombination system.
• Gene editing services
  Using the homologous recombination technique, two rox sites will be artificially constructed on the genome, and foreign genes with rox sites on both ends will be recombined into the genome. Also, we can perform specific gene activation analysis to understand the forward effect of the gene of interest.
• Dre-rox system modification services
  We can modify the dre element and rox element, and customize the new dre-rox system according to the customer's needs.

Case study: an advanced inducible Dre recombinant enzyme system

Nowadays, we have developed a highly efficient photo-activated Dre recombinase system. The Dre recombinase is split into two complementary N-terminal and C-terminal fragments. These two Dre fragments are brought together by a photo-activated dimerization system to achieve recombination.

• A comprehensive screening of potential cleavage sites in Dre primary sequences.
• The photo-activated Dre system was optimized and verified for recombinant activity.
• The activation detection of gene expression.
• Tumor genetic tracer analysis.

Creative Biolabs is your trusted partner. Our technical team possesses expertise and multidisciplinary capabilities, providing high-quality services. Our technical services are an ideal choice for tumor lineage tracing analysis. If you require Dre-rox recombination system-mediated lineage tracing services, please contact us or directly send us an inquiry. Our technical team will work closely with you to ensure a smooth project execution and help you achieve your research goals.

For Research Use Only | Not For Clinical Use