With advanced next-generation sequencing and mass spectrometry platforms, Creative Biolabs provides comprehensive multi-omics services and bioinformatics analysis. We have integrated microbiological, biochemical, and bioinformatic approaches to support neurotransmitters-related molecular measurement in the microbiota-gut-brain axis.
Host-associated microbiomes are emerging as important modifiers of brain activity and behavior. Metabolic, immune, and neuronal pathways are proposed to mediate communication across the so-called microbiota-gut-brain axis. Gut microbiota can either produce neurotransmitter precursors, catalyze the synthesis of neurotransmitters through dietary metabolism, or in combination. Some bacterial taxa may signal through their metabolites to promote the synthesis and release of neurotransmitters by enteroendocrine cells. Gut microbiota has been shown to produce and/or consume a wide range of mammalian neurotransmitters-related molecules, including tyrosine, dopamine (DA), norepinephrine (NE), serotonin, or gamma-aminobutyric acid (GABA), 5-hydroxytryptophan (5-HPT). These neurotransmitters by bacteria may have an impact on host physiology. Some neurotransmitters such as GABA cannot cross the blood-brain barrier and have localized effects within the gut. In contrast, other neuro-active molecules such as tyrosine can cross the blood-brain barrier and can affect both the gut and brain.
Fig.1 Gut microbial-mediated neurotransmitter synthesis and its impacts on cognition. (Chen, 2021)
The recent development in meta-analysis in combination with multiple technologies such as single-cell sequencing, 16s rDNA sequencing, mass spectrometry-based metabolomics including liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, and imaging mass spectrometry, has revealed potential links between gut microbiota and the metabolism and cognition of the host. Metabolomics can be applied to elucidate the link between the human microbiome and the metabolism of various cell types including the microbiota-gut-brain axis. Metabolomics approaches are ideal for quantitative analyses of neurotransmitter profiles.
Creative Biolabs provides a metabolomics analysis platform to support our clients to understand host and microbiome interactions, such as nontargeted and targeted analysis. Our comprehensive technology platform contains advanced instruments, including MS, GC-MS, LC-MS, NMR, and so on. Combining cutting-edge mass spectrometry with automated sample extraction, processing, and data analysis, we analyze hundreds of samples per day. We have extensive experience with the application and exploration of metabolomics across various studies. If you are interested in our services, please feel free to contact us for more information.
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