Precise quantification of bacterial loads is necessary for characterizing microbial dynamics. Quantitative PCR (qPCR)-based approach can be used to detect the absolute and relative abundances of gut microbiota. Scientists at Creative Biolabs provide qPCR analysis used to estimate microbial load or the agnostic quantification of microbiota in a sample.
The human gut microbiota has been considered the most important micro-ecosystem living in symbiosis with the body. A fundamental goal in microbiome studies is determining which microbes affect host physiology. High-throughput sequencing has emerged as one of the most important techniques for characterizing microbial dynamics and revealing bacteria and host interactions. Although the high-throughput technique is characterized by good accuracy and great sequencing depth, qPCR acts as a low-cost and high-efficiency alternative technique for the quantitative determination of target DNA abundance. q-PCR is widely used to determine values of absolute abundance, due to its robustness, sensitivity, and high reproducibility in recording quantitative changes of phylogenetic and functional gene markers across spatial and temporal scales. In addition, the qPCR-based approach can be used to detect the relative abundances of gut microbiota, thus determining changes in microbial taxa measure relative.
qPCR or flow cytometry has been used to estimate microbial load or the agnostic quantification of microbiota in a sample. With characteristics of flexibility and high sensitivity, qPCR-based methods are suitable for the prediction of accurate phylogenetic analysis. qPCR can detect taxonomy-specific targets but is limited in that targets for amplification must generally be prespecified. The appropriate primers provide great help to know the composition of a microbial community and microbial load.
16S rRNA genes in the bacterial genome can provide specific genetic information for distinguishing the bacterial taxa. PCR primers that match a specific gene, usually the 16S rRNA for bacteria and archaea and the internal transcribed spacer for fungi, are used to amplify all the variants that occur between the highly conserved regions used to construct the primers. In testing for gut microbial load, the 16S rRNA qPCR, a frequently used tool for gene expression detection and quantification in real-time, is commonly used for both absolute and relative quantification.
Based on our cutting-edge Gut Microbiota-Based Diagnostics Platform, Creative Biolabs supports a comprehensive gut microbial load analysis. Our absolute qPCR method for detecting bacterial abundance includes
Fig.1 The schematic chart for absolute qPCR for microbial determination in in vitro gastrointestinal digestion. (Zhang, 2020)
Creative Biolabs is dedicated to providing gut microbiota analysis and characterization services including culture-based analyses and qPCR testing. Our team includes experienced PhD-level molecular biologists, microbiome specialists and bioinformatics experts. We are pleased to support you through the full process, from design, through sample collection, data generation and processing, to the final analysis. If you are interested in our services, please feel free to contact us.
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