One-Stop Solution for Bispecific sdAb Development

During the past decades, a phenomenal evolution of bispecific antibodies (bsAbs) has been observed for both therapeutic and diagnostic applications. To meet the growing demand of bispecific antibodies, Creative Biolabs has established one-stop solutions to generate a series of novel bispecific antibodies which are based on the single domain antibody (sdAb). In terms of our extensive experience in sdAb discovery and development, our scientists are confident in developing bispecific sdAbs or other multivalent sdAb formats with high affinity and low immunogenicity for your projects.

Why Choosing Bispecific Single Domain Antibody or Other Multivalent sdAb Formats?

bsAb is an artificial protein that can simultaneously recognize two different epitopes either on the same or different antigens. In recent years, bispecific antibodies have become increasingly of interest for a broad spectrum of applications and been evaluated as potential treatments for a variety of indications, such as chronic inflammatory diseases, neurodegeneration, bleeding disorders, autoimmunity, cancers, and infections.

To further strengthen the advantages of bsAb, Creative Biolabs can employ sdAb as a specific source. sdAb is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in the conventional Fab region. Compared with convention antibodies, sdAb takes great advantages of its smaller size (~15 kDa), better penetrability, and high yield, which can be a better module for bispecific antibody design and manufacture. The conjugation of two or more sdAbs results in bivalent, trivalent, or even multivalent molecules with one or more specificities. For instance, two VHH molecules can be fused with a flexible linker derived from their native IgG format (from the hinge regions). Moreover, multivalent sdAb with one site directed against serum albumin can be a general approach for half-life extension, a similar idea can also contribute to delivering a target-specific component to pass the biological barriers.

Typical Features of Bispecific sdAb

Typical Pathway for Bispecific sdAb Development

Phase I Target Identification
Target Identification Multiple target identification processes can be provided upon your request.
Antigen Design & Preparation Starting with in silico sequence of targets, our scientists can handle the intact design and preparation processes to fit the demands for sdAb discovery. You can also provide the prepared target for further processes.
Premade Library Based sdAb Discovery Creative Biolabs has owned a series of high-quality in-house sdAb libraries (originated from camels, llamas as well as humans) which are great resources available for your sdAb discovery project.
Immunization Generation sdAb from different host animals (e.g. llama, alpaca, camel, and shark) with various immunization strategies (e.g. DNA immunization, whole cell immunization, and multiple sites immunization) to meet your specific demands.
Immune Library Based sdAb Discovery Our scientists are confident in generating high-quality immune library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.
Bispecific sdAb Construction Based on the selected sdAb candidates, bispecific or multivalent sdAb format can be properly designed against the targets.
Phase II Characterization
Functional sdAb Screening A series of assays are available to further analysis and screening sdAb candidates with desired functions, including but not limited to binding assays, immunomodulation assessments (activation or blocking), neutralizing assay, Fc profiling (ADCC, ADCP, CDC, etc.), endocytosis & internalization assays, cell proliferation assays.
sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).
sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase III Development
sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.
sdAb Humanization Creative Biolabs provides elegant sdAb humanization strategy, which is an efficient approach to reduce or eliminate the immunogenicity of sdAb drug candidates.
sdAb Conjugation A variety of custom sdAb conjugations are available of your choice.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase IV Manufacture
sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.
sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.

Features of One-Stop Bispecific sdAb Development

As an extremely fast-growing field, bispecific sdAb development presents great potentials for both therapeutic and diagnostic purposes. Creative Biolabs now can provide one-stop solutions to discover and develop the best-fit bispecific sdAbs for your purpose. If you are interested in learning more about our solutions, please do not hesitate to contact us.

We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.

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