One-Stop Solution for Anti-Idiotypic sdAb Development

Creative Biolabs has been long-term devoted to the development and application of novel single domain antibody (sdAb). Based on our advanced technology platform and extensive experience in anti-idiotypic sdAb discovery and development, our scientists are confident in setting up a one-stop solution to generate high-quality and specific anti-idiotypic sdAb for our customers all over the world.

Single domain antibody (sdAb) which recognizes the antigen-binding paratopes (idiotypes) presenting on the variable region of another antibody (known as Drug) is called anti-idiotypic (anti-ID) sdAb. Anti-idiotypic sdAb has four main types, including:

• Type 1 anti-ID sdAb can bind specifically to the paratope of Drug and inhibit the interaction of Drug and its target.
• Type 2 anti-ID sdAb can bind to the variable region of Drug but is not paratope specifically, which does not inhibit the binding of Drug and its target.
• Type 3 anti-ID sdAb can specifically recognize the complex formed by the interaction of Drug and its targets.
• Type 4 anti-ID sdAb can detect T cell receptors.

Potential Applications of Anti-ID sdAb

The facile identification of anti-ID sdAb, as well as their beneficial and unique properties (small size, high affinity, excellent stability, and low production cost), make it an ideal candidate for disease diagnosis and immunotherapy. Anti-ID sdAb can serve as anti-drug sdAb for drug candidate development and evaluation, such as pharmacokinetic/pharmacodynamics (PK/PD) studies, immunogenicity (immune response, IR) studies and antibody drugs enrichment and purification.

Anti-ID sdAb which can mimic the structure of the original antigen-binding site can be employed as antigen mimic sdAb for vaccine generation to elicit robust and specific immune responses and maintain the stable immune memory in the case where the original antigen is unavailable.

Anti-ID sdAb is of high value not only as a probe for studying cell receptors but also as an alternative reagent for immunoassays, as well as a new pathway for the development of abzyme. Anti-ID sdAb presents the potential to bear a structural copy of the enzyme active site due to their small size and thus mimics the function of the enzyme.

Features of Anti-ID sdAb

• The lower complexity and small size (~15kDa) allow mimicking small ligands and haptens
• Specifically bind to the antigen-binding region of the primary antibody
• Effectively mimic the three-dimensional structure of the original antigen (hidden/cleft epitopes)
• Competition with the original antigen for binding to the primary antibody
• Induction stable immune responses similar to the original antigen
• The excellent physical and thermal stability and resistance to stringent extreme conditions

Typical Pathway for Anti-Id sdAb Development

Phase I Discovery

	Antigen Design & Preparation Starting with in silico sequence of targets, our scientists can handle the intact design and preparation processes to fit the demands for sdAb discovery. You can also provide the prepared target for further processes.
Immunization Generation sdAb from different host animals (e.g. llama, alpaca, camel, and shark) with various immunization strategies (e.g. DNA immunization, whole cell immunization, and multiple sites immunization) to meet your specific demands.		Premade Library Based sdAb Discovery Creative Biolabs has owned a series of high-quality in-house sdAb libraries (originated from camels, llamas as well as humans) which are great resources available for your sdAb discovery project.
Polyclonal sdAb Discovery Our scientists are pleased to generate target-specific polyclonal antibody, including polyclonal camelid IgG (IgG1, IgG2, and IgG3), polyclonal heavy chain antibody (IgG2 and IgG3), or polyclonal sdAb (VHH).	Immune Library Based sdAb Discovery Our scientists are confident in generating high-quality immune library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.	B-Cell Sorting Based sdAb Discovery To bring our customers impeccable service of sdAb discovery, our seasoned scientific team has developed integrated B-cell sorting platform to maximize our customers' success in discovering valuable sdAbs.

Phase II Characterization

Functional sdAb Screening A series of assays are available to further analysis and screening sdAb candidates with desired functions, including but not limited to binding assays, immunomodulation assessments (activation or blocking), neutralizing assay, Fc profiling (ADCC, ADCP, CDC, etc.), endocytosis & internalization assays, cell proliferation assays.	sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).	sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase III Development

Multivalent sdAb Development The development of multivalent sdAb provides various alternative formats which can endow sdAbs with specific properties, such as extended half-life, increased avidity, and the recognition of different targets.	sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.	sdAb Humanization Creative Biolabs provides elegant sdAb humanization strategy, which is an efficient approach to reduce or eliminate the immunogenicity of sdAb drug candidates.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase IV Manufacture

sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.

sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.

Features of One-Stop Anti-Id sdAb Development

• The professional team with extensive experience in dealing with different types of anti-idiotypic sdAb
• Advanced technical platform to ensure the best achievement for every single step
• Fully customizable design to formulate the most suitable solutions
• Multiple pathways to enhance the success rate
• Reliable lab report with timely update
• Affordable price with the best quality

Creative Biolabs is a long-term expert focused on the discovery and development of novel sdAb. We are confident in providing the best service and the most suitable solution to satisfy your specific demands. If you are interested in developing high specific anti-idiotypic sdAb, please do not hesitate to contact us for more details.

Case Study

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sdAb Development for Soluble Protein Target

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sdAb Development for Hapten Target

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sdAb Development for 3 Targets via 1 Camelid Immunization

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sdAb Development via Premade HuSdL-1 Library

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sdAb Development for Low Immunogenic Target

All services are provided for research purposes only. Our services cannot be used for clinical or therapeutic applications.