One-Stop Solution for Anti-PTM sdAb Development

Creative Biolabs has been long-term devoted to the development and application of single domain antibody (sdAb). To fill the gaps in the field of PTMs identification and detection, our scientists have built one-stop solutions for anti-PTM sdAb development for our clients all over the world.

Discovery and Development of Novel Anti-PTM sdAb

Post-translational modification (PTM) refers to the covalent and generally enzymatic modification after the protein translation. It usually occurs on the amino acid side chains or at the C- or N- termini of protein with over 30 different classes (e.g. glycosylation, phosphorylation, and methylation). These various PTMs play key roles in a series of cellular processes, such as cell signaling, regulation of gene transcription, and protein degradation. These processes modulate the physical and chemical properties, conformation, folding, stability, and activity of proteins, thereby modifying the functions of proteins and even may lead to cancers. In this case, the identification and detection of PTMs are critical to understand cell biology as well as disease mechanisms and further manipulate the biological mechanisms of protein.

Currently, characterization of PTMs heavily relies on proteomics analyses and anti-PTM antibodies have been served as an essential component for detecting and enriching PTMs. Consequently, the discovery and development of highly specific and potent antibodies to PTMs become very urgent to meet the needs of the market. Single domain antibody has now become a better option than conventional antibody for PTM recognition because of its unique properties of smaller size (~15 kDa) and better penetrability. sdAb is expected to recognize PTM sites more specifically, which can improve the efficiency to identify the target PTM and reduce background caused by cross-reaction. With over ten years of extensive experience, Creative Biolabs can provide customized solutions to generate high specific sdAbs for different PTM sites.

Typical Pathway for PTM-specific sdAb Development

Phase I Target Identification

PTM Peptides Peptides can be designed and synthesized properly to mimic the desired PTM sites. Carrier proteins will be conjugated for short peptides to ensure immunogenicity or promote screening processes.

Antigen Design & Preparation Starting with in silico sequence of targets, our scientists can handle the intact design and preparation processes to fit the demands for sdAb discovery. You can also provide the prepared target for further processes.

PTM Protein Custom protein production service can be tailored to generate target protein with desired post-translational modification, which can be used for the validation stage.

Phase II Discovery

Polyclonal sdAb Discovery Our scientists are pleased to generate target-specific polyclonal antibody, including polyclonal camelid IgG (IgG₁, IgG₂, and IgG₃), polyclonal heavy chain antibody (IgG₂ and IgG₃), or polyclonal sdAb (V_HH).

Immunization Generation sdAb from different host animals (e.g. llama, alpaca, camel, and shark) with various immunization strategies (e.g. DNA immunization, whole cell immunization, and multiple sites immunization) to meet your specific demands.

B-Cell Sorting Based sdAb Discovery To bring our customers impeccable service of sdAb discovery, our seasoned scientific team has developed integrated B-cell sorting platform to maximize our customers' success in discovering valuable sdAbs.

Immune Library-Based Discovery Our scientists are confident in generating high-quality immune library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.

Premade Library Based sdAb Discovery Creative Biolabs has owned a series of high-quality in-house sdAb libraries (originated from camels, llamas as well as humans) which are great resources available for your sdAb discovery project.

Phase III Characterization

Functional sdAb Screening A series of assays are available to further analysis and screening sdAb candidates with desired functions, including but not limited to binding assays, immunomodulation assessments (activation or blocking), neutralizing assay, Fc profiling (ADCC, ADCP, CDC, etc.), endocytosis & internalization assays, cell proliferation assays.	sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).	sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase IV Development

Multivalent sdAb Development The development of multivalent sdAb provides various alternative formats which can endow sdAbs with specific properties, such as extended half-life, increased avidity, and the recognition of different targets.	sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.	sdAb Conjugation A variety of custom sdAb conjugations are available of your choice.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase V Manufacture

sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.

sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.

Features of One-Stop Anti-PTM sdAb Development

Extensive experience in dealing with a variety of PTMs
Advanced technical platform
Fully customizable design to meet every specific demand
Fast turnaround time
Reliable lab report with timely update
Affordable price with the best quality

Creative Biolabs is a leading service provider that focuses on novel sdAb discovery and development. We are pleased to share our extensive experience and advanced platform to satisfy each of your specific demands. If you are interested in generating high specific anti-PTM sdAbs, please do not hesitate to contact us for more details.

Case Study

Download now

One-Stop Solution for Anti-PTM sdAb Development

Discovery and Development of Novel Anti-PTM sdAb

Typical Pathway for PTM-specific sdAb Development

Features of One-Stop Anti-PTM sdAb Development

Case Study

sdAb Development for Soluble Protein Target

sdAb Development for Hapten Target

sdAb Development for 3 Targets via 1 Camelid Immunization

sdAb Development via Premade HuSdL-1 Library

sdAb Development for Low Immunogenic Target