One-Stop Solution for Neutralizing sdAb Development

Neutralizing sdAb presents a crucial therapeutic opportunity against virus or toxin infections. Creative Biolabs has years’ experience in the field of sdAb development; our scientists can provide the best-quality one-stop solution for the development of neutralizing sdAb to offer the best support to help advance our clients' programs.

Virus or toxin neutralization by antibodies predominantly relies on the ability to block the interaction between the virion and a specific cellular receptor, thereby blocking viral entry and release. Such blocking may occur due to direct binding to a viral epitope involved with receptor interaction. In order to treat or prevent infectious diseases or poisoning by protein toxins, sdAb with blocking effect and neutralizing capacity could be a small potential drug/inhibitor candidate for prophylactic and therapeutic treatment.

Neutralizing sdAb

The current limitations in diagnosis and therapeutic applications have forced to think for a new antibody format with better stability, faster tissue distribution, higher binding affinity, and neutralizing capacities of toxin and virus. sdAb is the smallest natural single-antigen-binding fragment (~15 kDa) which possesses many attractive features such as the ability to identify and bind a wide variety of epitopes especially hidden viral epitopes; the capacity to refold following thermal or chemical denaturation; ease of cloning and large-scale production; a large degree of homology with the human VH, and rapid tissue penetration as well as rapid clearance. The convex conformations formed of sdAbs can target unique and cryptic epitopes and confer unique binding specificities by blocking the concave epitopes of antigens. Moreover, their small size makes them ideal building blocks for the generation of multivalent or multispecific constructs to increase affinity and neutralizing potency and prevent the appearance of viral escape mutants. More importantly, sdAbs can be easily administered via the inhalation or oral route, directly to the site of viral infection because of their small size and favorable biophysical characteristics, making them considerable advantages for the treatment, such as the fast onset of action, low systemic exposure, and high concentration at the site of disease.

Some neutralizing sdAbs specifically recognize toxins or viruses without cross-reactivity with closely related structures of the members of the family. In contrast, there is a group of broad neutralizing sdAb, which can bind to the functionally conserved epitopes of a series of related targets. Based on our advanced technology platforms and extensive experience, Creative Biolabs has set up a one-stop procedure to develop not only the specific neutralizing sdAb but also the broad neutralizing sdAb with the highest efficiency and the best quality for any targets. These sdAbs can also be easily engineered to further increase neutralization activity by increasing binding moieties, such as multivalent constructs. The sdAb-mediated neutralization against viruses or toxins is significant to improve neutralizing sdAb-based diagnostics, engineered vaccines, and new small molecule therapeutic strategies to prevent recurrent infections.

Typical Pathway for Neutralizing sdAb Development

Phase I Discovery
Antigen Design & Preparation Starting with in silico sequence of targets, our scientists can handle the intact design and preparation processes to fit the demands for sdAb discovery. You can also provide the prepared target for further processes.
Immunization Generating sdAb from different host animals (e.g., llama, alpaca, camel, and shark) with various immunization strategies (e.g., full-length antibody, Fab fragment, or scFv fragment for immunization) to meet your specific demands.
B-Cell Sorting Based sdAb Discovery To bring our customers impeccable service of sdAb discovery, our seasoned scientific team has developed integrated B-cell sorting platform to maximize our customers' success in discovering valuable sdAbs.
Immune Library Based sdAb Discovery Our scientists are confident in generating high-quality immune library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.
Premade Library Based sdAb Discovery Creative Biolabs has owned a series of high-quality in-house sdAb libraries (originated from camels, llamas as well as humans) which are great resources available for your sdAb discovery project.
Phase II Characterization
sdAb Neutralization Assay Our scientists offer the best-fit neutralization assays in order to characterize and analysis of the sdAb candidates with desired neutralizing activity.
sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).
sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase III Development
Multivalent sdAb Development The development of multivalent sdAb provides various alternative formats which can endow sdAbs with specific properties, such as extended half-life, increased avidity, and the recognition of different targets.
sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.
sdAb Humanization Creative Biolabs provides elegant sdAb humanization strategy, which is an efficient approach to reduce or eliminate the immunogenicity of sdAb drug candidates.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase IV Manufacture
sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.
sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.


Features of One-Stop Neutralizing sdAb Development

The potent neutralizing sdAb has shown promise for future diagnostic, prophylactic, or therapeutic applications. With our well-established platform and rich experience, Creative Biolabs can offer the best one-stop service and high-quality neutralizing sdAbs to contribute to the successes of your projects significantly. Please feel free to contact us for more information and a detailed quote.

We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.

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