One-Stop Solution for sdAb Specificity Optimization

Creative Biolabs is a well-recognized expert in the field of sdAb discovery and development. With our rich experience of phage display mutant library technology and in silico modeling, Creative Biolabs is now offering a novel service to optimize the specificity of the sdAb of interest, which can solve your trouble from unwanted cross-reaction.

Remove Cross-Reaction or Enhance Target Specificity of sdAb Candidates

In general, sdAbs bind their targets with highly specific selectivity, which is a prerequisite for their use in targeted therapy. However, due to the sequence similarity of some proteins, sdAb against specific targets may have poor specificity or even cross-reacts to other unwanted proteins with high homology. A functional candidate may have to be abandoned when it cannot meet the required specificity for use in intended applications.

Recent advances in the sequence analysis and computer modeling of sdAb fragments have created opportunities to solve this problem. Taken the advantages of deep understanding of antibody repertoire and in silico modeling, Creative Biolabs has explored novel solutions through rationally designed engineering processes to achieve the improvement of binding specificity or elimination of unwanted cross-reactions. Starting with in silico sequence of the sdAb of interest, its target, and the unwanted crossed protein(s), our scientists can investigate the three-dimensional binding structure and design sdAb candidates to obtain optimized specificity against the desired targets without cross-reaction. This approach is particularly desirable for therapeutically promising sdAbs that suffer from insufficient specificity toward target antigen or unwanted cross-reactivity, which avoids many of the side effects associated with off-target perturbations.

Typical Pathway for sdAb Specificity Optimization

Phase I Discovery
Sequence Analysis Starting with in silico sequence of primary sdAbs and targets, our scientists can handle the intact design and prediction processes to fit the demands for sdAb specificity optimization.
In Silico Modeling Our scientists can use in silico modeling to generate a 3D structure for mutation design for the best-fit candidate sites.
Mutant Library Based sdAb Discovery Our scientists are confident in generating a high-quality mutant library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.
Phase II Characterization
sdAb Specificity Verification Creative Biolabs can verify the specificity of selected sdAb upon to your specific demands.
sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).
sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
Functional sdAb Screening A series of assays are available to further analysis and screening sdAb candidates with desired functions, including but not limited to binding assays, immunomodulation assessments (activation or blocking), neutralizing assay, Fc profiling (ADCC, ADCP, CDC, etc.), endocytosis & internalization assays, cell proliferation assays.
sdAb Paratope Mapping Paratope, also named as an antigen-binding site, is a part of the antibody which allows the recognition and binding to an antigen. Paratope mapping is a powerful technology to understand the antigen-binding site well and plays an important role in vaccines and biotherapeutics discovery.
sdAb Epitope Mapping Epitopes consist of groups of amino acids which interact with antibodies and therefore determine the antigenicity. Now we can provide the epitope mapping services with 100% accuracy and efficiency.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase III Development
Multivalent sdAb Development The development of multivalent sdAb provides various alternative formats which can endow sdAbs with specific properties, such as extended half-life, increased avidity, and the recognition of different targets.
sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.
sdAb Humanization Creative Biolabs provides elegant sdAb humanization strategy, which is an efficient approach to reduce or eliminate the immunogenicity of sdAb drug candidates.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.
Phase IV Manufacture
sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.
sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.


Features of One-Stop sdAb Specificity Optimization

In terms of our advanced technical platforms, scientists at Creative Biolabs are confident in offering high-quality sdAb specificity optimization solutions to assist your valuable projects. A series of sdAb candidates can be rapidly optimized with desired specificity to meet your particular application requirements. Should you be interested in this novel specificity optimization service, please feel free to contact us for more details.

We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.

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