Specific Single Domain Antibody (SdAb) Characterization Services
Along with over a decade of extensive experience in providing excellent custom services for sdAb characterization, Creative Biolabs has won great reputation among our worldwide customers for successfully accomplishing numerous challenging sdAb characterization projects. Equipped with advanced platforms and powerful technologies, we are confident in offering the most suitable solution and achieving the best outcome for our customers.
With the efficacy of either antigen-binding region or fused Fc region, sdAb can present various functions. Creative Biolabs offers a full range of functional assays to further analysis and screening sdAb candidates with desired functions.
Typical Function Assays
Binding assay
Cell proliferation assay
Immunomodulation assessment (activation or blocking)
Equipped with our proprietary database assisted shotgun sequencing (DASS) technology, Creative Biolabs now provides the soluble and functional sdAbs sequencing services with 100% coverage and 100% accuracy. The DASS system provides reliable supporting proof for QC analysis, IND/NDA application, and confirmation & validation of produced antibodies.
Typical Features
Fast & Sensitive: The full coverage can be obtained from 50-200 μg sdAbs within 10 working days.
High-Throughput: Our DASS method enables to plan your project with 100, 200, 500 or more sdAbs.
sdAb Affinity Measurement
Based on the powerful optical phenomena technologies: bio-layer interference (BLI) and surface plasmon resonance (SPR), Creative Biolabs provides the sdAb affinity measurement service with our advanced ProteOn, Biacore, Octet systems, etc.
SPR is an extremely sensitive method which can monitor the binding of any two unlabeled molecules in real time. Combining the sensorgram data with a mathematic model, we are able to calculate the association (Kon ) and dissociation (Koff ) rate constants and finally the binding affinity (KD).
BLI is an optical analytic approach to determine the biomolecular mutual effect. Apart from the antibody affinity measurement, BLI is also a powerful tool for fragment screening in drug discovery.
Two of the principal measurable parameters relating to sdAb stability are physical stability (thermodynamic stability) and chemical stability (proteolytic stability). sdAbs with high stability are desirable for therapeutics and vaccines. Now we can provide various stability analytical approaches for our customers.
Stability Evaluation Strategies
Dynamic light scattering (DLS)
Differential scanning calorimetry (DSC)
Differential scanning fluorimetry (DSF)
Liquid chromatography-mass spectrometry (LC-MS)
High-performance liquid chromatography (HPLC)
Paratope Mapping
Paratope, also named as an antigen-binding site, is a part of the antibody which allows the recognition and binding to an antigen. Paratope mapping is a powerful technology to understand the antigen-binding site well and plays an important role in vaccines and biotherapeutics discovery.
Paratope Mapping Strategies
Computational procedure
Hydrogen-deuterium exchange and mass spectrometry
Peptide-based approaches
Hopfield network
Epitope Mapping
Epitopes consist of groups of amino acids which interact with antibodies and therefore determine the antigenicity. Now we can provide the epitope mapping services with 100% accuracy and efficiency.
Epitope Mapping Strategies
Peptide microarray
Limited proteolysis coupled with mass spectrometry
Amide hydrogen/deuterium exchange
Nuclear magnetic resonance (NMR) analysis
Surface plasmon resonance
X-ray crystallography
Alanine scanning mutagenesis
Microfluidic peptide microarray
Surface display methods
Developability Assessment
The unsatisfactory physical and chemical properties of drug molecules result in huge cost during the therapeutic protein discovery. Now, Creative Biolabs has built the comprehensive CreDA™ developability assessment service to avoid the potential clinical failure for your biopharmaceutical candidates.
Our CreDA™ platform consists of two major modules: the preliminary screening stage and the comprehensive assessment stage, which enable the choice of the most appropriate lead molecular for your further processes.
Developability Assessment Strategies
Preliminary screening
Comprehensive assessment
Manufacturability assessment
Immunogenicity assessment of drug candidates
In vivo fitness assessment
Based on our integrated platform and professional science teams, Creative Biolabs is always dedicated to assisting our clients with the most satisfactory sdAb characterization services. If you are interested in our service, please feel free to contact us more details.
Reference
Henry, Kevin A., et al. "Stability-diversity tradeoffs impose fundamental constraints on selection of synthetic human VH/VL single-domain antibodies from in vitro display libraries." Frontiers in immunology 8 (2017): 1759. Distributed under Open Access license CC BY 4.0, without modification.
