One-Stop Solution for sdAb Development

Creative Biolabs has been a long-term expert in the field of single domain antibody (sdAb) development. Based on our advanced technology platforms and extensive experience, we have won a good reputation for successfully accomplishing numerous challenging sdAb development projects. Currently, our seasoned scientists have set up a series of one-stop procedures which can assist your sdAb development with the highest efficiency and the best quality.

High Throughput Procedure for Novel sdAb Development

Along with over a decade of extensive experience in antibody development, Creative Biolabs can develop novel sdAb to meet our clients’ various project requirements. From the target identification to the final sdAb production, our one-stop solution can offer a series of pathways to achieve the project goals, which can free up your valuable time and effort on the innovative side of the project.

Typical Pathway for Target-specific sdAb Development

Phase I Discovery

Target Identification Multiple target identification processes can be provided upon your request.		Antigen Design & Preparation Starting with in silico sequence of targets, our scientists can handle the intact design and preparation processes to fit the demands for sdAb discovery. You can also provide the prepared target for further processes.		Premade Library Based sdAb Discovery Creative Biolabs has owned a series of high-quality in-house sdAb libraries (originated from camels, llamas as well as humans) which are great resources available for your sdAb discovery project.
Polyclonal sdAb Discovery Our scientists are pleased to generate target-specific polyclonal antibody, including polyclonal camelid IgG (IgG1, IgG2, and IgG3), polyclonal heavy chain antibody (IgG2 and IgG3), or polyclonal sdAb (VHH).		Immunization Generation sdAb from different host animals (e.g. llama, alpaca, camel, and shark) with various immunization strategies (e.g. DNA immunization, whole cell immunization, and multiple sites immunization) to meet your specific demands.		Immune Library Based sdAb Discovery Our scientists are confident in generating high-quality immune library and performing comprehensive library screening and validation strategies to select the best-fit sdAbs for your targets.
		B-Cell Sorting Based sdAb Discovery To bring our customers impeccable service of sdAb discovery, our seasoned scientific team has developed integrated B-cell sorting platform to maximize our customers' success in discovering valuable sdAbs.

Phase II Characterization

Functional sdAb Screening A series of assays are available to further analysis and screening sdAb candidates with desired functions, including but not limited to binding assays, immunomodulation assessments (activation or blocking), neutralizing assay, Fc profiling (ADCC, ADCP, CDC, etc.), endocytosis & internalization assays, cell proliferation assays.	sdAb Affinity Measurement A series of technologies are available to measure the affinity of specific sdAbs with the most advanced systems (e.g. Biacore, ProteOn, Octet).	sdAb Stability Evaluation A series of custom analytical approaches are available to assess the stability of specific sdAbs upon your request, including the evaluation of aggregation and stability under extreme condition.
More Creative Biolabs offers a variety of sdAb characterization services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase III Development

Multivalent sdAb Development The development of multivalent sdAb provides various alternative formats which can endow sdAbs with specific properties, such as extended half-life, increased avidity, and the recognition of different targets.	sdAb Affinity Maturation Elaborate procedures can be tailored to develop sdAbs with increased affinity against the target of interest.	sdAb Conjugation A variety of custom sdAb conjugations are available of your choice.
More Creative Biolabs offers a variety of sdAb development services for your project. Our scientists can also provide customized solutions to meet your specific demands.

Phase IV Manufacture

sdAb Scale-up Production Transient transfection method can be employed for custom sdAb production via either E. coli or mammalian system.

sdAb Stable Cell Line Construction Our scientists are professional in constructing stable cell lines with high yield to meet your demands of long-term sdAb production.

Applications of sdAb

In terms of the unique properties of small size, high stability, and outgoing solubility, single domain antibody enables researchers and scientists to advance in a variety of fields, which including but not limited to:

• The study of protein structure, aggregation and activity regulation
• The identification and targeting of hapten, bacteria, phages, fungi, and protozoans
• The detection and neutralization of virus
• The identification and detoxification of toxin
• For high-resolution imaging applications [such as multiphoton imaging, nuclear imaging conjugated, and Near-infrared (NIR) imaging]

Features of One-Stop sdAb Development

• Comprehensive procedures and experimental details
• Advanced technical platform
• Experienced professional team
• Fully customizable design to meet every specific demand
• Fast turnaround time
• Reliable lab report with timely update
• Affordable price with the best quality

Creative Biolabs has long-term devoted to the development and application of sdAbs. Our scientists are confident in offering the best and most suitable solution for our customers all over the world. If you are considering developing novel sdAbs against the target of interest, please do not hesitate to contact us for more details.

Case Study

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sdAb Development for Soluble Protein Target

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sdAb Development for Hapten Target

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sdAb Development for 3 Targets via 1 Camelid Immunization

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sdAb Development via Premade HuSdL-1 Library

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sdAb Development for Low Immunogenic Target

We are offering highly customized CRO services to assist your Single Domain Antibody (sdAb) related projects. Please Contact Us for more details.