All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
Anti-CD19 h(BBζ) CART, Combined with Dasatinib represents a CAR Plus system, which can be used in the research of Acute myeloid leukemia. In the system, the transduced CAR lentiviral vector was constructed to express scFv of an anti-CD19 antibody linked to the CD28-CD3ζ signaling domains.The CAR Plus system combined Dasatinib to improve the antitumor activity and reduce CRS in adoptive immunotherapy. Creative Biolabs provides customized CAR Plus system design and construction services. Please contact us for more detailed information.
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28ζ Fig.1 Leukaemic response and relapse patterns associated with FMC63 scFv-based CAR. Kaplan-Meier survival analysis of mice bearing NALM6 cells treated with 0.2 × 10^6 transduced CAR T cells. n = 5-7 mice per group pooled from two independent experiments. Hamieh, M., Dobrin, A., Cabriolu, A., van der Stegen, S. J., Giavridis, T., Mansilla-Soto, J., ... & Sadelain, M. (2019). CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape. Nature, 568(7750), 112-116. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28ζ Fig.2 Leukaemic response and relapse patterns associated with FMC63 scFv-based CAR. Representative FACS profile of PD-1, LAG-3 and TIM-3 surface expression on BBζ CAR T cells isolated from relapsed mice. (data are representative of n = 3 mice per group). Hamieh, M., Dobrin, A., Cabriolu, A., van der Stegen, S. J., Giavridis, T., Mansilla-Soto, J., ... & Sadelain, M. (2019). CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape. Nature, 568(7750), 112-116. |
CAR Construction : FMC63 scFv-28ζ Fig.4 FMC63 and 4G7 CAR T cells effectively responded to CD19‐positive NALM‐6 cells. Tumor-killing ability of FMC63, 4G7, and FITC CAR T cells against luciferase-expressing NALM-6 and U937. FMC63, 4G7, and FITC CAR T cells (2 × 10^5 cells) were co-cultured with luciferase-expressing NALM-6 (2 × 10^4 cells) or U937 (2 × 10^4 cells) for indicated incubation times at the E:T ratio 10:1. Then, luminescence values were measured. Kang, C. H., Kim, Y., Lee, H. K., Lee, S. M., Jeong, H. G., Choi, S. U., & Park, C. H. (2020). Identification of potent CD19 scFv for CAR T cells through scFv screening with NK/T-cell line. International Journal of Molecular Sciences, 21(23), 9163. |
CAR Construction : Fig.5 Kinetic binding properties of CD19 scFv derived from CD19 hybridomas. Anti-CD19 scFv was fused to mouse IgG2a-Fc in a SFG-eBFP γ-retroviral expression vector and HEK293T cells were transfected to generate secreted scFv, which were then purified Ghorashian, S., Kramer, A. M., Onuoha, S., Wright, G., Bartram, J., Richardson, R., ... & Amrolia, P. J. (2019). Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR. Nature medicine, 25(9), 1408-1414. |
CAR Construction : FMC63 scFv-BBζ Fig.6 Antigen-specific killing of CD19+ tumor cells by CD19 CAR+ T cells (FMC63 or CAT) or non-transduced (NT) T cells. CAR+ T cell cytotoxic activity against SupT1 cells that are engineered to express CD19 (SupT1CD19, left) as well as target-antigen-negative non-transduced SupT1NT (right), as measured by standard 4-h 51Chromium-release assay. Ghorashian, S., Kramer, A. M., Onuoha, S., Wright, G., Bartram, J., Richardson, R., ... & Amrolia, P. J. (2019). Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR. Nature medicine, 25(9), 1408-1414. |
CAR Construction : FMC63 scFv-BBζ Fig.7 Cytokine production in response to 1:1 coculture of FMC63-CAR cells with irradiated Raji cells. Production of cytokines in response to 1:1 coculture of CAR cells with irradiated Raji cells, measured by a cytokine bead array of culture supernatants Ghorashian, S., Kramer, A. M., Onuoha, S., Wright, G., Bartram, J., Richardson, R., ... & Amrolia, P. J. (2019). Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR. Nature medicine, 25(9), 1408-1414. |
CAR Construction : FMC63 scFv-BBζ Fig.8 FMC63 CAR-T cells show effector-specific function with wildtype CD19+ targeted cells in vitro. Tumor cells were incubated with CAR-T cells for 6 hours. Then the tumor cells were collected and stained with Annexin-V/PI to determine specific lysis. Zhang, Z., Chen, X., Tian, Y., Li, F., Zhao, X., Liu, J., ... & Zhang, Y. (2020). Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy. Journal for immunotherapy of cancer, 8(2). |
CAR Construction : FMC63 scFv-BBζ Fig.9 FMC63 CAR-T cells show effector-specific function with mutant CD19+ targeted cells in vitro. Tumor cells were incubated with CAR-T cells for 6 hours. Then the tumor cells were collected and stained with Annexin-V/PI to determine specific lysis. Zhang, Z., Chen, X., Tian, Y., Li, F., Zhao, X., Liu, J., ... & Zhang, Y. (2020). Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy. Journal for immunotherapy of cancer, 8(2). |
CAR Construction : FMC63 scFv-BBζ Fig.10 Bioluminescent imaging of antitumor ability of CD19 CAR-T cells in vivo. 1e6 luciferase-transgenic Raji cells were inoculated into SCID-beige mice via tail on day 7. The 1e6 wild type or mutated CD19 positive H322/Luc cells were subcutaneously injected into the mice. On day 0, 1e7 CAR-Τ cells were injected intravenously (intravenous). Then, tumor growth was monitored via bioluminescent imaging test. Zhang, Z., Chen, X., Tian, Y., Li, F., Zhao, X., Liu, J., ... & Zhang, Y. (2020). Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy. Journal for immunotherapy of cancer, 8(2). |
CAR Construction : FMC63 scFv-BBζ Fig.11 Bioluminescent imaging of antitumor ability of CD19 CAR-T cells in vivo. 1e6 luciferase-transgenic Raji cells were inoculated into SCID-beige mice via tail on day 7. The 1e6 wild type or mutated CD19 positive H322/Luc cells were subcutaneously injected into the mice. On day 0, 1e7 CAR-Τ cells were injected intravenously (intravenous). Then, tumor growth was monitored via bioluminescent imaging test. Zhang, Z., Chen, X., Tian, Y., Li, F., Zhao, X., Liu, J., ... & Zhang, Y. (2020). Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy. Journal for immunotherapy of cancer, 8(2). |
CAR Construction : FMC63 scFv-BBζ Fig.12 Data summary of antitumor ability of CD19 CAR-T cells in vivo. 1e6 luciferase-transgenic Raji cells were inoculated into SCID-beige mice via tail on day 7. The 1e6 wild type or mutated CD19 positive H322/Luc cells were subcutaneously injected into the mice. On day 0, 1e7 CAR-Τ cells were injected intravenously (intravenous). Then, tumor growth was monitored via bioluminescent imaging test. Zhang, Z., Chen, X., Tian, Y., Li, F., Zhao, X., Liu, J., ... & Zhang, Y. (2020). Point mutation in CD19 facilitates immune escape of B cell lymphoma from CAR-T cell therapy. Journal for immunotherapy of cancer, 8(2). |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.13 Anti-tumor activities of mFMC63-CAR T cells. Fluorescence images of HeLa-CD19+ expressing mCherry co-cultured with CAR T cells at an E:T ratio of 10:1 as analyzed by fluorescence microscopy. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.14 Anti-tumor activities of mFMC63-CAR T cells. Bar graphs represent the percentages of cytotoxicity against HeLa-CD19+ target cells by CAR T cells as measured by crystal violet staining assay. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.15 Anti-tumor activities of mFMC63-CAR T cells. Cytotoxicity of Raji cells expressing CD19 antigen by CAR T cells after co-culturing at an E:T ratio of 1:1 for 24 and 48 h. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.16 IL-2 production in response to coculture of FMC63-CAR cells with Raji cells. Levels of secreted IL-2 cytokines in the culture supernatants of co-cultured CAR T cells and Raji cells. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.17 IFN-γ production in response to coculture of FMC63-CAR cells with Raji cells. Levels of secreted IFN-γ cytokines in the culture supernatants of co-cultured CAR T cells and Raji cells. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-BBζ; FMC63 scFv-28BBζ Fig.18 TNF-α production in response to coculture of FMC63-CAR cells with Raji cells. Levels of secreted TNF-α cytokines in the culture supernatants of co-cultured CAR T cells and Raji cells. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-28BBζ Fig.19 The killing activities of mFMC63-CAR4 T cells against Raji cell line. The killing activities of mFMC63-CAR4 T cells against Raji (CD19+) cells after co-culturing at effector to target (E:T) ratios of 1:1, 2.5:1, and 5:1 for 4 hours. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-28BBζ Fig.20 The killing activities of mFMC63-CAR4 T cells against K562-CD19. The killing activities of mFMC63-CAR4 T cells against K562-CD19+ cells after co-culturing at effector to target (E:T) ratios of 1:1, 2.5:1, and 5:1 for 4 hours. Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-28BBζ Fig.21 IFN-γ production in the cell culture supernatants of anti-CD19 CAR T cells after activation by culturing with Raji (CD19+) cells. The levels of IFN-γ production in the cell culture supernatants of anti-CD19 CAR T cells after activation by culturing with Raji (CD19+) cells at an E:T ratio of 5:1 for 24 h were analyzed by enzyme-linked immunosorbent assay (ELISA). Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
CAR Construction : FMC63 scFv-28BBζ Fig.22 TNF-α production in the cell culture supernatants of anti-CD19 CAR T cells after activation by culturing with Raji (CD19+) cells. The levels of TNF-α production in the cell culture supernatants of anti-CD19 CAR T cells after activation by culturing with Raji (CD19+) cells at an E:T ratio of 5:1 for 24 h were analyzed by enzyme-linked immunosorbent assay (ELISA). Wutti-In, Y., Sujjitjoon, J., Sawasdee, N., Panya, A., Kongkla, K., Yuti, P., ... & Yenchitsomanus, P. T. (2021). Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains. Frontiers in oncology, 11. |
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