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Anti-FRα (353.2-1)-CD3ζ CAR-MA (CARMA-W0741)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-FRα chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human FRα and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-FRα antibody (353.2-1) linked to CD3ζ signaling domains. The vector product was designed for the treatment of Ovarian, urothelial bladder carcinoma.

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Details

  • Target
  • FRα
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Ovarian, urothelial bladder carcinoma
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • 353.2-1
  • Hinge
  • CD8
  • TM
  • CD8
  • ICD
  • CD3ζ
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(CD8)-TM(CD8)-ICD(CD3ζ)
  • Discription of Signaling Cassetes
  • CD3ζ:
    CD3ζ is used as the CAR intracellular signaling domain. CD3ζ has homology to the Fc common γ-chain, FcεRI-γ, a canonical signaling molecule for antibody_x005f_x005f_x005F_x0002_dependent cellular phagocytosis (ADCP) in macrophages. CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

  • Target
  • FRα
  • Target Species
  • Human
  • Clone
  • 353.2-1
  • Host
  • Mouse
  • Gene Name
  • Folate receptor alpha
  • Introduction
  • The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
  • Alternative Names
  • FBP; FOLR; NCFTD; FRalpha; FOLR1; Folate receptor alpha

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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