Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-14 Plex (CAR-K-LX-106)

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Product Name
Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-14 Plex

Cat. No.
CAR-K-LX-106

Product Summary
Panel enables the study of T helper response by analyzing 14 protein targets in a single well using Luminex xMAP technology, which can simultaneous detect and quantity up to 14 protein targets in a single 25-50 µL sample-from plasma, serum, cell culture supernatants, and other bodily fluids.

Components
2 vials Rat Standard Mix A (lyophilized)
1 vial Capture Bead Mix (1X)
1 vial Biotinylated Detection Antibody Mix (50X)
1 bottle Reading Buffer (1X)
1 bottle Wash Buffer (10x)
1 bottle Streptavidin-PE (1X)
1 bottle Universal Assay Buffer (1X)
1 bottle Detection Antibody Diluent (1X)
8-tube strip
Adhesive film
Flat-bottom 96-well plate, black

Precision
Intra-assay: <15
Inter-assay: <15

Applications
Simultaneously analyze multiple cytokine and chemokine and other biomarkers with Bead-Based Multiplex Assays using the Luminex technology.

Product format/Physical form
96-well microplate

Assay Type
Multiplex

Cross-reactivity
There was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.

Species Reactivity
Rat

Sample Type (General)
Serum, Plasma, Cell Culture Supernatants

Shipping Condition
The product is recommended to be shipped on dry ice.Upon receipt, store immediately at recommended temperature below.

Storage Conditions
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Plex size
14

Dtection Coverage
G-CSF (CSF-3), GM-CSF, IFN gamma, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A (CTLA-8), TNF alpha

Performance Validation
Our Luminex Assays undergo our most extensive validation testing. All Luminex Assays are validated for use with serum and plasma samples and tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types.

Detection method
Fluorescent

Product Features
Reproducible, reliable results-validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing;
More results per sample-measure multiple protein targets simultaneously in a single 25-50 µL sample;
Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation;

Principle of the Assay
Based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Research Category
Inflammation & Immunology

Application Notes
This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples.

Accuracy
>80%

Panel Type
MAGNETIC Cytokines/Chemokines/Other Markers

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Rat Magnetic Luminex Mutiplex Cytokine/Chemokine Array Kit-14 Plex (CAR-K-LX-106)

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