All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
Sleeping Beauty (SB) transposon, a type of nonviral integrative vectors, provides an alternative to modify primary T cells. Creative biolabs has developed SB transposon CAR vector pSBCAR1 TAG72 (huCC49) h(ICOSBBζ), which is constructed for the engineering of T cells to target human TAG72. The T cells are genetically modified through transduction with a nonviral vector expressing scFv of anti-TAG72 antibody linked to ICOS (CD278) signaling domain and CD137 (4-1BB), CD3-zeta signaling domains. And the vector product was designed for the treatment of Ovarian cancer.
CAR Construction : huCC49 scFv-41BB-CD3ζ Fig.1 TAG72-specific CAR T cells containing a 4-1BB intracellular co-stimulatory domain. Mock (untransduced) and TAG72-BBζ CAR T cells were evaluated by flow cytometry for CD19t expression to detect lentiviral transduction of CARs (left) or Protein L to detect the scFv (right). Murad, J. P., Kozlowska, A. K., Lee, H. J., Ramamurthy, M., Chang, W. C., Yazaki, P., Colcher, D., Shively, J., Cristea, M., Forman, S. J., & Priceman, S. J. (2018). Effective Targeting of TAG72+ Peritoneal Ovarian Tumors via Regional Delivery of CAR-Engineered T Cells. Frontiers in immunology, 9, 2268. |
CAR Construction : huCC49 scFv-41BB-CD3ζ Fig.2 TAG72-BBζ CAR T cells show antigen-dependent cytokine production and tumor killing in vitro. Quantification of tumor killing and (H) IFNγ production by TAG72-BBζ CAR T cells relative to Mock following a 72 h co-culture with freshly thawed OAS cells at an E:T ratio of 1:1. Murad, J. P., Kozlowska, A. K., Lee, H. J., Ramamurthy, M., Chang, W. C., Yazaki, P., Colcher, D., Shively, J., Cristea, M., Forman, S. J., & Priceman, S. J. (2018). Effective Targeting of TAG72+ Peritoneal Ovarian Tumors via Regional Delivery of CAR-Engineered T Cells. Frontiers in immunology, 9, 2268. |
CAR Construction : huCC49 scFv-41BB-CD3ζ Fig.3 Regional intraperitoneal delivery of TAG72-BBζ CAR T cells significantly reduces tumor burden and extends overall survival of OVCAR3 tumor-bearing mice. Kaplan-Meier survival for Mock and TAG72-BBζ CAR T cell treated mice. N ≥ 4 mice per group. Combined data from two independent experiments. Murad, J. P., Kozlowska, A. K., Lee, H. J., Ramamurthy, M., Chang, W. C., Yazaki, P., Colcher, D., Shively, J., Cristea, M., Forman, S. J., & Priceman, S. J. (2018). Effective Targeting of TAG72+ Peritoneal Ovarian Tumors via Regional Delivery of CAR-Engineered T Cells. Frontiers in immunology, 9, 2268. |
More Published Data More Published Data
There are currently no customer reviews or questions for Anti-TAG72 (huCC49) h(ICOS-4-1BB-CD3ζ) CAR, pSBCAR1 (CAR-SB-02LX668). Click the button below to contact us or submit your feedback about this product.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
For any technical issues or product/service related questions, please leave your information below. Our team will contact you soon.
The latest newsletter to introduce the latest breaking information, our site updates, field and other scientific news, important events, and insights from industry leaders
LEARN MORE NEWSLETTERCellRapeutics™ In Vivo Cell Engineering: One-stop in vivo T/B/NK cell and macrophage engineering services covering vectors construction to function verification.
LEARN MORE SOLUTIONSilence™ CAR-T Cell: A novel platform to enhance CAR-T cell immunotherapy by combining RNAi technology to suppress genes that may impede CAR functionality.
LEARN MORE NOVEL TECHNOLOGYCanine CAR-T Therapy Development: From early target discovery, CAR design and construction, cell culture, and transfection, to in vitro and in vivo function validation.
LEARN MORE SOLUTION