A498 Cell Proliferation Assay for Immune-Oncology Field

A498 cells, derived from human kidney carcinoma, serve as an ideal model to study tumor proliferation and the impact of various treatments. Notably, the A498 proliferation assay is instrumental in elucidating the mechanisms of action of potential immunotherapeutic targeting the kidney cancer microenvironment, thereby aiding in the development of more effective cancer treatments.

A498 Cell Proliferation Assay at Creative Biolabs

At Creative Biolabs, we leverage our extensive experience of over two decades in providing cutting-edge biological assays, including the A498 cell proliferation assay. Our team of experts ensures the highest assay fidelity and reliability, customized to meet the specific needs of our clients in the biotech and pharmaceutical sectors. For A498 cell proliferation experiments, we offer a variety of experimental types to meet the diverse needs of our customers' projects. For example, for preclinical studies, we offer tailored high-throughput screening-based proliferation assay mode to deliver scaled outcomes. Our adept professionals employ state-of-the-art technologies to deliver precise and reproducible results.

Our Available Assay Types

Creative Biolabs provides a broad spectrum of A498 cell-based assays designed to cater to various research requirements. These include but are not limited to:

• Functional Cell-Based Assays: Assessing the biological relevance of test compounds through cell viability and proliferation measures.
• Luminescence-Based Proliferation Assays: Utilizing luminescent markers to provide sensitive and quantitative readouts of cell proliferation.
• Transwell Invasion Assays: Evaluating the invasive potential of cancer cells post-treatment with test compounds.

Highly Featured Luminescence-Based A498 Proliferation Assay

Our luminescence-based A498 proliferation assay stands out for its remarkable sensitivity and specificity. This method employs a luminescent detection mechanism to quantify cell viability with exceptional precision. The assay hinges on the detection of ATP, a marker of metabolically active cells, thus providing a direct correlation with cell proliferation.

Assay Details

• Control Inhibitor: Staurosporine is employed as a reference inhibitor to validate assay performance.
• Criteria for Significance: A ≤ 50% reduction in cell growth signifies substantial activity.
• Test Concentrations: Compounds are screened across a gradient of concentrations, ranging from 0.01 μM to 30 μM.
• Sample Requirements: Minimal quantities of the test sample are required, ensuring the efficient use of valuable compounds.

Scientific Backing

Empirical evidence underpins the efficacy of the A498 proliferation assay.

Frequently Asked Questions

Q1: How does the A498 proliferation assay differentiate between cytostatic and cytotoxic effects?

A1: The assay distinguishes cytostatic effects, which inhibit cell growth, from cytotoxic effects, which kill cells, by comparing post-treatment cell counts to both initial seeding and untreated controls using parameters like GI50, TGI, and LC50.

Q2: Can the assay be modified for other cancer cell lines?

A2: Yes, Creative Biolabs offers customizable options to adapt the proliferation assay to various cancer cell lines, ensuring relevance to specific research needs.

Creative Biolabs' A498 cell proliferation assay represents a pivotal tool in the immuno-oncology arena, providing insightful data for novel therapeutic development. Our comprehensive and customizable assay services, backed by scientific rigor and detailed protocols, ensure unparalleled precision and efficacy in advancing cancer research.

Reference

1. Chen, Yuan-Min, et al. "Ursolic acid inhibits the invasiveness of A498 cells via NLRP3 inflammasome activation." Oncology Letters 20.5 (2020): 1-1. Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use