B Cell based Nuclear Magnetic Resonance (NMR) Assay Service
Creative Biolabs offers a comprehensive and precise view of drug-target interactions. By utilizing this powerful, label-free approach, we provide quantitative and physiologically relevant data directly within a native cellular context. This capability is absolutely crucial for significantly de-risking your drug development process and dramatically accelerating your timeline from initial discovery to a successful and well-understood therapeutic candidate. Our goal is to empower your team with actionable insights that lead to better, more effective drugs.
Introduction What We Can Offer Workflow Why Creative Biolabs Customer Reviews FAQs Related Services Contact Us
Antigen-antibody Interaction NMR Spectrum
Nuclear magnetic resonance (NMR) spectroscopy is a sensitive and high-resolution technique that can be used to characterize antigen-antibody interactions. NMR is a widely used structure technique for small, less complicated molecule analysis and can yield high-resolution, multidimensional spectra of large molecules. The NMR technique is necessary to help us further understand the antigen-antibody interaction dynamics and obtain predominant antigen epitope information.
With extensive knowledge of structural biology and antigen epitope discovery, Creative Biolabs offers the antibody-based NMR Assay for cancer epitope analysis for clients all over the world. For a detailed discussion on how our services can advance your project, request a consultation.
Fig.1 Simplified diagram of a nuclear magnetic resonance spectrometer.1
What We Can Offer
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What We Can Offer
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Generate 3D solution structures of a protein.
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Monitor the dynamic behavior of the molecule.
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Measure dynamic parameters about antibody-antigen interaction.
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Insight into the structural detail of the antibody interaction with the primary peptide and conformational epitope.
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NMR Assay Service at Creative Biolabs
Creative Biolabs offers NMR spectroscopy technique-based epitope mapping by comparing the NMR spectrum changes before and after an antibody binds to an antigen and determining the atoms at the interaction interface.
Highlights
High Sensitivity
Our assay offers superior sensitivity to detect even weak, transient interactions that traditional methods often miss. This capability significantly increases your hit rate and improves drug lead compound screening efficiency, accelerating your early-stage discovery process.
High Resolution
We provide atomic-level detail for binding site mapping and conformational changes. This high-resolution insight is crucial for rational drug design and lead optimization, allowing you to fine-tune compounds for improved potency and selectivity.
Multidimensional Analysis
Our multidimensional NMR analysis provides a comprehensive view of your drug-target interaction. We capture key data points like kinetics and stoichiometry, giving you a complete mechanistic understanding beyond simple binding.
Reduced Costs
By providing physiologically relevant and high-quality data early, our service helps you reduce protein expression costs by up to 50%. This also de-risks your project, preventing costly failures in later development stages.
Discover how a partnership with us can streamline your research - Get a quote today.
Customer Reviews
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Clear Insights
Using Creative Biolabs' assay in our research has significantly improved our ability to distinguish true binders from non-specific interactions, saving us months of validation work. We were particularly impressed with the detailed kinetic data provided. - La K*, R&D Scientist.
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Physiological Relevance
Creative Biolabs' service facilitated the identification of a novel allosteric binding site on our membrane protein target. The data proved that our lead compound's mechanism of action was entirely different from what was predicted by our in vitro assays. - Jt B*, Principal Investigator.
FAQs
Why should I choose a B cell-based NMR assay over a conventional cell-based assay?
Conventional assays often rely on indirect readouts and can be prone to artifacts from labels or artificial conditions. Our B cell-based NMR assay provides a direct, label-free, and highly quantitative look at your drug-target interaction in a more physiologically relevant context, giving you higher-quality, more reliable data.
Is your B cell-based NMR Assay limited to a specific type of target protein?
No, our service is highly versatile. We specialize in challenging targets, including membrane proteins and those requiring specific post-translational modifications. Our team can work with you to engineer the appropriate B cell line for your specific target.
Related Services
High-Throughput Antibody Production Platform
Our high-throughput platform provides end-to-end antibody development, from discovery to purification. We utilize advanced technologies like phage display, hybridoma, and single B-cell platforms to ensure rapid, scalable, and efficient production of high-quality antibodies for your project's needs.
Learn More →
Protein Purification & Development
We provide comprehensive and customized services for protein purification, including process development and optimization. Our solutions ensure high-quality and scalable production of a wide range of proteins and antibodies, meeting your specific research needs.
Learn More →
How to Contact Us
Creative Biolabs is dedicated to protein structure discovery using multiple methods, including NMR assay and X-ray crystallography assay, to push the antibody-based therapy development for cancer disease. We promise a reliable, reproducible, and accurate result on cancer epitope analysis. Please contact us for further discussion of your project with our experts.
Reference
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Rankin, Naomi J et al. "The emergence of proton nuclear magnetic resonance metabolomics in the cardiovascular arena as viewed from a clinical perspective." Atherosclerosis vol. 237,1 (2014): 287-300. Distributed under an Open Access license CC BY 3.0, without modification. https://doi.org/10.1016/j.atherosclerosis.2014.09.024
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Pourmodheji, Hossein et al. "A Multidisciplinary Approach to High Throughput Nuclear Magnetic Resonance Spectroscopy." Sensors (Basel, Switzerland) vol. 16,6 850. 9 Jun. 2016. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/s16060850
For Research Use Only | Not For Clinical Use
