Creative Biolabs offers Human Brain Cell DBTRG-05MG-based Proliferation Assays which are used to measure the rate of cell growth and division in the DBTRG-05MG human brain cell line.

DBTRG-05MG Human Brain Cells

DBTRG-05MG-based Proliferation Assay at Creative Biolabs

This assay was conducted on DBTRG-05MG human brain cells to assess the rate of cell proliferation in response to various treatments or conditions. In this assay, DBTRG-05MG cells are seeded and treated with the compound to be tested. After a specified incubation period, a luminescent substrate was added to the wells, which reacted with ATP produced by metabolically active cells to produce light. The results show that the luminescence signal intensity is proportional to the number of viable cells in the well.

Assay Information

Assay Procedure

Our assay demonstrates the utility of a luminescence-based method for assessing tumor cell proliferation in DBTRG-05MG human brain cancer cells. This method provides a sensitive and reliable assay for evaluating the efficacy of anti-cancer compounds and may help in the development of new therapeutic strategies for brain cancer treatment.

• Process details
  • Prepare cell culture: DBTRG-05MG human brain cells should be cultured in an appropriate growth medium in a cell culture incubator at 37°C with 5% CO₂.
  • Seed cells in 96-well plates: After reaching approximately 70-80% confluence, trypsinize the cells and seed them in 96-well plates at a desired cell density.
  • Treat cells with compounds: Treat the cells with different compounds or experimental treatments at various concentrations. Include appropriate controls in the assay.
  • Incubate cells: Incubate the cells with the compounds for a specified period of time at 37°C in the cell culture incubator.
  • Measure cell proliferation: After the incubation period, measure cell proliferation using a luminescence assay kit.
  • Measure luminescence: After incubating cells at room temperature to stabilize the luminescence signal, measure the luminescence signal using a microplate reader.
  • Analyze the data: Calculate the percentage of cell proliferation in each treatment condition compared to the control. Plot the results as a dose-response curve to determine the IC50.

Frequently Asked Question

Q1: Why use DBTRG-05MG cells for the proliferation assay?

A1: DBTRG-05MG cells are commonly used for proliferation assays because they are a well-characterized glioblastoma cell line that has been extensively studied in research settings. These cells are known to have a high proliferation rate, which makes them suitable for assessing the effects of potential anti-proliferative compounds or treatments. Additionally, DBTRG-05MG cells are easy to culture and maintain in the laboratory, making them a convenient choice for proliferation assays. Overall, the use of DBTRG-05MG cells in proliferation assays can provide reliable and consistent results that can help researchers better understand the mechanisms of cell growth and proliferation.

Contact Us

If you have any questions about our DBTRG-05MG Human Brain Cell Tumor Cell Proliferation Assay or would like to inquire about pricing and ordering, please feel free to reach out to us. Trust Creative Biolabs to always put your project first!

For Research Use Only | Not For Clinical Use