Introduction

The transcription factor nuclear factor kappa B (NF-κB) is integral to numerous cellular processes, predominantly in immunity and inflammation. It is pivotal in the pathogenesis of various diseases, ranging from cancer to neurological disorders like Alzheimer's disease. At the cellular level, NF-κB regulates gene expression critical for immune response, cell survival, and apoptosis. Recognizing its significance, Creative Biolabs has developed a state-of-the-art NFκB quantification service, catering to the diverse needs of modern biological research and clinical diagnostics.

Fig.1 Schematic of NF-κB signaling pathway. ¹

NFκB Quantification Service at Creative Biolabs

Creative Biolabs offers a cutting-edge NFκB quantification service designed to measure NF-κB activation with unparalleled precision. Our services leverage the latest technological advances to provide accurate, reproducible results across various biological samples, including tissues, cell lysates, and live cells. Utilizing multiple quantification methodologies ensures that we can address the broad spectrum of research and clinical needs effectively.

Service Content

Our NFκB quantification service encompasses the following techniques:

• Phosphorylation-Specific Assays

NF-κB activation is often monitored through its phosphorylation. Our innovative ELISA-based assays quantify NF-κB activation by detecting the phosphorylation states of NF-κB subunits. This high-throughput technique ensures accurate measurement even under pathophysiological conditions such as inflammation and cancer.

• Western Blotting

Widely used in protein research, Western blotting provides quantification of various NF-κB subunits' abundance and activity states. This semi-quantitative method is pivotal for understanding signal transduction pathways from activation to post-translational modifications.

• Immunostaining

Using immunohistochemistry and immunofluorescence, Creative Biolabs can localize NF-κB subunits within tissue samples or cultured cells. This technique, despite its challenges, offers invaluable anatomical detail, thus contributing to a comprehensive understanding of NF-κB's role.

Highlights of Our Service

• Precision and Sensitivity: Our methodologies ensure high detection sensitivity, crucial for studying low-abundance proteins like NF-κB in different cellular contexts.
• Comprehensive Analysis: Integration of multiple assays provides a holistic view of NF-κB activity, from protein-DNA interactions to transcriptional regulation.
• Expertise: Our team of experienced scientists ensures meticulous execution of assays, leveraging over two decades of industry knowledge.
• Customization: Tailor-made solutions to fit the specific needs of our clients, whether in basic research, clinical diagnostics, or drug development.
• State-of-the-Art Equipment: Utilization of cutting-edge instrumentation and technologies for optimal assay performance.

Applications of Our NFκB Quantification Service

The accurate quantification of NF-κB activity finds applications across diverse biomedical fields:

• Cancer Research: Monitoring NF-κB activity in cancer cells helps understand tumor biology and the role of inflammatory pathways in tumor progression.
• Neuroscience: Investigating NF-κB signaling in the central nervous system provides insight into neurological disorders, such as understanding its role in synaptic plasticity and neuroinflammation.
• Immunology: Quantifying NF-κB activation in immune cells elucidates the mechanisms underlying immune responses and autoimmune diseases.
• Drug Development: Screening for NF-κB inhibitors and evaluating their efficacy in preclinical studies.
• Inflammation Studies: Investigating NF-κB-regulated pathways in inflammatory diseases, such as rheumatoid arthritis and psoriasis.

Frequently Asked Questions

Q1: What is the significance of NF-κB in biological processes?

A1: NF-κB is a critical transcription factor involved in regulating immunity, inflammation, cell proliferation, apoptosis, and various other cellular processes.

Q2: What types of samples are compatible with Creative Biolabs' NFκB Quantification Service?

A2: Our service is compatible with a wide range of samples, including cell lysates, tissue extracts, primary cells, and cultured cell lines. We also offer specialized protocols for delicate samples like neuronal tissues and clinical biopsies.

Creative Biolabs remains committed to advancing scientific research by providing top-notch NFκB quantification services. Whether you are exploring fundamental biological processes or seeking new therapeutic targets, our comprehensive and reliable assays will meet your research needs effectively.

Reference

1. Mostafizar, Marina, et al. "Challenges with methods for detecting and studying the transcription factor nuclear factor kappa B (NF-κB) in the central nervous system." Cells 10.6 (2021): 1335. Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use