p53 Assay Service
Background Service Workflow Highlights Contact Us
To facilitate discovery programs focused on cancer immunology and tumor microenvironment modulation, Creative Biolabs provides a versatile p53 Assay Service platform tailored for quantitative assessment of p53 pathway activity, protein stability, transcriptional function, and interactions with downstream effector genes.
Background
p53, a pivotal tumor suppressor protein encoded by the TP53 gene, serves as a central node in cellular stress responses such as DNA damage, oncogene activation, and hypoxia. Beyond its classical roles in cell cycle arrest, apoptosis, and senescence, emerging evidence underscores p53's relevance in tumor immune surveillance, immune checkpoint regulation, and cytokine signaling. Mutations or functional inactivation of p53 are highly prevalent in a wide spectrum of human cancers, making it an essential biomarker and functional target for oncology research and therapeutic development.
Featured Services at Creative Biolabs
Our p53 assay services enable researchers to interrogate p53 status and modulation using a variety of readouts, supporting the development of small molecules, siRNAs, and immuno-oncology strategies. Core services include:
Reporter Gene Assays
Luciferase or GFP-based systems driven by p53-responsive promoters for high-throughput transcriptional activity analysis.
p53 Protein Stability Assays
Detection of endogenous or tagged p53 under proteasome inhibition, stress conditions, or compound treatment.
Western Blot & ELISA Quantification
Isoform-specific detection of p53 and key regulators (e.g., MDM2, p21, BAX, PUMA).
Phosphorylation/Acetylation Status Analysis
PTM profiling under genotoxic or oxidative stimuli to assess pathway activation.
Immune-Linked Readouts
Evaluation of p53-mediated transcription of immune-related genes (e.g., IFNB1, IL12, PD-L1) in tumor-immune crosstalk models.
We support both cell-based and biochemical assay formats, adaptable to different species (human, mouse) and cell lines, including wild-type and mutant p53 backgrounds.
Workflow
We follow a modular, yet customizable workflow to meet diverse assay development needs:
Details:
Experimental Design Consultation
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Define project goals, cell model selection, detection method, and stimulation conditions.
Assay Setup & Optimization
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Optimize reporter system, protein detection, or transcript quantification for target sensitivity.
Stimulation or Compound Treatment
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Apply stressors or test candidates in defined conditions.
Data Collection & Analysis
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Quantify p53 levels, transcriptional activity, or downstream pathway markers.
Report Delivery
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Clean, interpretable datasets with raw data, fold-change metrics, and statistical outputs.
We offer full flexibility for pathway-specific assays, isoform differentiation, and custom readout design.
Highlights
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Comprehensive p53 Pathway Readouts – From transcriptional response to protein dynamics
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Immune-Relevant Outputs – Integration with inflammatory and checkpoint markers
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Wild-Type & Mutant p53 Compatibility – Tools for functional restoration or inhibition studies
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Flexible Detection Platforms – Reporter, ELISA, Western blot, and qPCR-based solutions
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Ideal for Immuno-Oncology Research – Suited for screening, mechanistic validation, and target discovery
Accelerate Your Tumor Immunology Projects with Expert p53 Assay Solutions
Creative Biolabs offers comprehensive, end-to-end support for both academic and industrial research teams investigating the pivotal role of the p53 axis in cancer immunity. Leveraging our extensive expertise in molecular biology, immuno-oncology, and advanced assay development, we provide tailored solutions ranging from target validation and pathway analysis to functional screening and biomarker discovery. Our flexible service portfolio ensures that each project can be customized to meet specific experimental goals, timelines, and regulatory requirements. Contact us today to discuss your research needs, explore assay customization options, or request a detailed quote to accelerate your p53-focused cancer immunity studies.
