Creative Biolabs specializes in providing a proliferation assay based on human breast cells T-47D. This particular assay is designed to measure the rate at which these cells divide and grow over a period of time. By studying the proliferation of these cells, you can gain valuable insights into the behavior and function of breast cells in both normal and disease states. Our assay is highly accurate, reliable, and reproducible, making it an essential tool for studying breast cancer, drug screening, and other research applications.

T-47D Cell Line

T-47D-based Proliferation Assay at Creative Biolabs

The Human Breast Cell T-47D-based Proliferation Assay is a method commonly used to measure the proliferation of tumor cells in vitro. In this assay, T-47D cells are seeded in 96-well plates and treated with desired compounds or conditions. After a predetermined incubation period, a luminescent substrate is added to the wells, which produces a proportional signal to the number of viable cells present. Luminescence is then measured, and the results are used to assess the effect of different treatments on cell proliferation. This assay can be useful for studying the effects of various factors on breast cancer cell growth, such as drug treatments, gene knockdowns, or growth factor stimulation.

Assay Information

Assay Procedure

Our assay is typically performed using T-47D breast cancer cells, which have been engineered to express a luciferase reporter gene that produces light when the cells are actively dividing.

• The T-47D cells are cultured in a growth medium containing essential nutrients and growth factors.
• The T-47D cells are treated with various compounds or conditions to study their effects on cell proliferation. This can include adding growth factors, hormones, drugs, or other experimental treatments.
• After treatment, luciferase substrates, such as luciferin, are added to the cells. The luciferase enzyme produced by the cells reacts with the substrate to generate light, which can be measured using a luminometer.
• The luminescence produced by the T-47D cells is then quantified using a luminometer.

Applications of Our Assay

• Drug screening

The T-47D-based proliferation assay can be used to screen potential drugs for their ability to inhibit or promote cell proliferation.

• Toxicity testing

The assay can also be used to evaluate the toxic effects of various chemical compounds on T-47D cells.

• Mechanistic studies

The T-47D-based proliferation assay can be used to study the molecular mechanisms underlying cell proliferation in breast cancer.

• Biomarker discovery

The assay can be used to identify potential biomarkers of breast cancer progression or drug response.

Frequently Asked Questions

Q1: Why use T-47D cells for the proliferation assay?

A1:

• T-47D cells exhibit positivity for hormone receptors, particularly estrogen and progesterone receptors, making them suitable for the study of hormone-responsive proliferation mechanisms.
• T-47D cells have a moderate growth rate, making them suitable for studying proliferation rates over a short period of time.
• The molecular and genetic characteristics of T-47D cells have been extensively studied, making them a reliable and consistent model for proliferation assays.
• T-47D cells can be easily cultured in the laboratory and are readily available from cell repositories, making them convenient for researchers to work with.

Contact Us

If you are interested in using a human breast cell T-47D-based proliferation assay for immuno-oncology research, you can contact Creative Biolabs for this service. We can assist you in designing, performing, and analyzing analyses to meet your specific research needs.

For Research Use Only | Not For Clinical Use