Introduction

Human THP-1 cells are an extensively used in vitro model to study immune modulation, particularly in relation to monocyte and macrophage functions. This human leukemia monocytic THP-1 cell line helps simulate crucial biological responses, making them invaluable in pharmaceutical and nutraceutical research. Creative Biolabs, leveraging over 20 years of expertise in the industry, has developed cutting-edge approaches and assays using THP-1 cells to enhance our understanding of cell proliferation, signaling pathways, cellular interactions, and immune responses.

THP-1-based Proliferation Assay at Creative Biolabs

At Creative Biolabs, we leverage the THP-1 cell-based proliferation assay to advance cancer drug discovery and evaluate immune-modulating compounds. Our assay paradigms provide robust and reliable data that correlate closely with in vivo responses. We offer an extensive range of cell proliferation and viability assays to meet diverse research needs, including but not limited to some of the following:

• Luminescence Assay: For rapid screening with a colorimetric readout, enhancing throughput and reproducibility.
• BrdU Incorporation Assay: Measures DNA synthesis during cell division, offering highly precise cell proliferation data.
• MTT, and WST-8 Assays: Directly measure metabolic activity, correlating well with cell viability.
• Real-Time Kinetic Measurement via High-content Analysis: Allows real-time tracking of cellular proliferation dynamics.
• 3D Tumor Spheroid Assays or Soft Agar Assays: adaptable to extended incubation periods.

Robust Luminescence-based THP-1 Proliferation Assay Empower Immune-oncology Field

One of the standout assays at Creative Biolabs is the luminescence-based THP-1 proliferation assay. Utilizing a luminescent readout, this assay measures cell viability and proliferation in a highly sensitive and quantitative manner. The assay's robustness is evident in its wide applicability, from screening anti-cancer agents to investigating the effects of bioactive substances on immune cells.

Luminescence-based THP-1 Proliferation Assay Procedure

The procedure begins with the seeding of THP-1 cells at an optimized concentration in a 96-well plate, followed by overnight incubation in a controlled atmosphere of 5% CO₂ at 37 °C. Post overnight incubation, test compounds or controls are introduced for a further 72 hours or other special incubation periods. Luminescence measurement, conducted using a microplate reader, determines the growth inhibition metrics – GI50 and TGI, alongside the LC50, providing a multi-faceted view of cell proliferation and viability.

Benefits for You

Collaborating with Creative Biolabs on THP-1-based proliferation assays offers multiple advantages:

Frequently Asked Questions

Q1: What makes the THP-1 cell line ideal for proliferation assays?

A1: The THP-1 cell line closely mimics the behavior of human monocytes and macrophages, making it highly relevant for studying immune responses, drug efficacy, and mechanistic pathways in vitro.

Q2: Can Creative Biolabs assist with long-term drug incubation studies?

A2: Yes, we offer 3D Tumor Spheroid Assay and Soft Agar Assay services that allow for drug incubation periods extending beyond three days, suitable for long-term studies.

Q3: Can the THP-1 proliferation assay be used for high-throughput screening?

A3: Absolutely. Our Luminescence-based THP-1 Proliferation Assay is designed for high-throughput capabilities, accommodating multiple compounds simultaneously using 96-well, 384-well plates, thereby efficiently supporting extensive research projects.

For more information on our THP-1-based proliferation assays and other services, contact our global business development team today and let Creative Biolabs support your research needs with unparalleled expertise and service quality.

For Research Use Only | Not For Clinical Use