Human Brain Cell U-87 MG-based Proliferation Assay Service
The U-87 MG cell line is a commonly used human glioblastoma cell line that is often used in cancer research. Creative Biolabs has recently launched a proliferation assay service based on human brain cells U-87 MG to help you evaluate the effect of test compounds on tumor cell proliferation.
U-87 MG-based Proliferation Assay at Creative Biolabs
Our U-87 MG-based cell proliferation assay service utilizes U-87 MG cells to assess the growth and proliferation of cells in response to various treatments or conditions. This service offers a reliable and quantitative method for measuring cell viability, growth kinetics, and drug efficacy, providing valuable insights into the effects of compounds on cell proliferation and potential therapeutic applications. The U-87 MG-based assay is ideal for drug screening, toxicity testing, and basic research purposes, offering high sensitivity and reproducibility to meet research and development needs.
Assay Information
Assay Procedure
Our U-87 MG-based proliferation assay typically performs as follows:
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Cell Culture: Cultivate U-87 MG human brain cells in an appropriate cell culture medium supplemented with fetal bovine serum and antibiotics. Maintain the U-87 MG cells in a humidified incubator at 37°C and 5% CO2.
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Treatment: Plate the cells in a 96-well plate at a desired cell density and treat them with your test compounds or substances of interest. Include appropriate controls such as vehicle-treated cells.
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Cell Proliferation Assay: After the treatment period, assess cell proliferation using a suitable method such as a colorimetric assay, luminescence-based assay, or a cell counting meth.
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Data Analysis: Calculate the percentage of cell proliferation in treated cells compared to untreated control cells.
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Repeat the assay with different concentrations of the test compounds to establish dose-response relationships and determine the half-maximal inhibitory concentration (IC50) if applicable.
For this assay, it is critical to optimize the assay conditions and controls to ensure reliable and reproducible results. Additionally, consider validating the findings in other cell lines or in vivo models to confirm the anti-proliferative effects of the test compounds on tumor cells.
Applications of Our Assay
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Drug screening
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Toxicity testing
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Cancer research
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Genetic studies
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Biomarker discovery
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By measuring the proliferation of U-87 MG cell lines in the presence of different drug compounds, you can identify potential candidates for further testing.
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By measuring the effects of samples on cell proliferation, you can determine their potential harmful effects on human health.
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By studying the proliferation of U-87 MG cell lines, you can gain insights into the mechanisms of cancer growth and potential therapeutic targets.
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By manipulating gene expression in U-87 MG cell lines and measuring their proliferation, you can elucidate the functions of various genes in the context of cancer and other diseases.
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By measuring the proliferation of U-87 MG cell lines in response to different stimuli, you can identify molecular markers that are altered in diseased cells compared to healthy cells.
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Frequently Asked Questions
Q1: What is the project timeline for a proliferation assay based on U-87 MG?
A1: The time for the U-87 MG-based proliferation assay can vary depending on the specific protocol used. However, it typically ranges from 3-6 weeks, during which the cells are treated with a compound of interest and their proliferation is measured at different time points.
Q2: Why use U-87 MG cells for the proliferation assay?
A2: U-87 MG cells are commonly used in proliferation assays because they are a well-characterized human glioblastoma cell line that grows rapidly and can be easily maintained in culture. They have been extensively studied and are known to exhibit reliable and consistent growth rates, making them a suitable model for studying cell proliferation. Additionally, U-87 MG cells are widely available and have been used in many research studies, allowing for comparison of results across different experiments.
Contact Us
We are here to assist with any inquiries you may have regarding our proliferation assay service based on human brain cells U-87 MG. If you have any questions or would like to learn more about our proliferation analysis services, please contact us today. Our experts in the field of immuno-oncology are ready to address your concerns and provide you with any assistance you may need. Thank you for considering us for your proliferation assay needs.
For Research Use Only | Not For Clinical Use
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