Ultrafiltration is a pressure-driven purification process in which water and low-molecular-weight substances permeate a membrane while colloids, particles, and macromolecules are retained. Creative Biolabs provides ultrafiltration assays to remove endotoxins from water, salts, and small molecule therapeutics. Here we specifically focus on the ultrafiltration system and our proprietary ultrafiltration cartridges are used to removing endotoxin from ultrapure water.
The control and removal of endotoxin are based on the principle of endotoxin-binding peptide purification resin, which needs to be controlled from the beginning of protein preparation experiments. All practical utensils, instruments, water, reagents, and pipettes with no endotoxin are used for endotoxin removal. Moreover, all the experiments are performed in a cleanroom, ensuring that all levels of low endotoxin tests were achieved.
Creative Biolabs offers ultrafiltration assay and unique ultrafiltration cartridges to meet the specific needs of different customers.
The project is conducted in a cleanroom within a negative-pressure isolator for safety reasons. Samples are held at 3±2°C and first passed through a sterile 0.22-um PVDF filter to remove the bioburden. Two polyethersulfone ultrafiltration cassettes are used in parallel to remove the endotoxin within the stability window at 5°C while minimizing dead volumes. This step is run as a dead-end filtration at gauge pressures of 1.4-3.1 bar for 6 h with an average flux of 9 LMH/bar. The experimental setup is shown as follows.
Fig.1 Experimental setup. (De, 2015)
Ultrapure water can harbor some endotoxin-producing bacteria even if it is considered to be an inhospitable environment. Autoclaving or filtration may be used to remove bacteria, but few sterilization methods can destroy the rest of the endotoxins.
Creative Biolabs offers ultrafiltration cartridges containing thin polysulfone hollow fibers designed to remove endotoxins and other macromolecules from water. Working with endotoxin-containing cultures may lead not only to inaccurate or erroneous results but also to loss of time, money, and effort. However, placing an ultrafiltration cartridge at the water purification system's point of use will eliminate this potential risk.
Creative Biolabs guarantees that all instruments, water, reagents, and consumables used in the experiment are free of endotoxins, and the experiment is conducted in a cleanroom to ensure that low levels of endotoxins are returned to the sample. you can purchase the related accessory products according to the needs of your endotoxin removal project. For more detailed information, please feel free to contact us or directly sent us an inquiry.
References
For Research Use Only | Not For Clinical Use
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