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Anti-Fetal AChR (mAb192)-CD147 CAR-MA (CARMA-W0703)


All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The vector of anti-Fetal AChR chimeric antigen receptor (CAR) is constructed for the engineering of macrophage cells to target human Fetal AChR and induce target cell phagocytosis. The macrophage cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-Fetal AChR antibody (mAb192) linked to CD147 signaling domains. The vector product was designed for the treatment of Osteosarcoma, rhabdomyosarcoma.

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Details

  • Target
  • Fetal AChR
  • Cell Type
  • Macrophage
  • Targeting Diseases
  • Osteosarcoma, rhabdomyosarcoma
  • Vector Name
  • pCDCAR1
  • Vector length
  • ~8kb
  • Vector Type
  • Lentiviral vector
  • Size
  • 10 µg
  • Storage
  • Store at -20°C for long term.

CAR Components

  • Promoter
  • EF1a
  • scFv
  • mAb192
  • Hinge
  • IgG1
  • TM
  • CD147
  • ICD
  • CD147
  • Marker
  • GFP or N/A
  • Receptor Construction
  • scFv-hinge(IgG1)-TM(CD147)-ICD(CD147)
  • Discription of Signaling Cassetes
  • To breakdown the "physical barrier" of the tumour matrix, CD147 is used as the signaling domain. It's a membrane molecule that is essential for ECM remodelling via the expression of MMPs.

Target

  • Target
  • Fetal AChR
  • Target Species
  • Human
  • Clone
  • mAb192
  • Host
  • Rat
  • Gene Name
  • Fetal acetylcholine receptor
  • Introduction
  • This gene encodes a member of a family of proteins that are receptor associated proteins of the synapse. The encoded protein contains a conserved cAMP-dependent protein kinase phosphorylation site, and plays a critical role in clustering and anchoring nicotinic acetylcholine receptors at synaptic sites by linking the receptors to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Mutations in this gene may play a role in postsynaptic congenital myasthenic syndromes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • Alternative Names
  • FADS; CMS11; CMS4C; FADS2; RAPSYN; RNF205; RAPSN; Receptor associated protein of the synapse; fetal AChR

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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