Canine CAR-T Engineering Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs specializes in offering canine CAR-T engineering services to speed up global clients' projects.

The Rational of Canine CART

Canine serves as an excellent model for human disease. There are over 300 diseases that have been described as similar to human disease, including several cancers such as leukemia, lymphoma, osteosarcoma, and melanoma. Moreover, canine tumors occur spontaneously, which recapitulates the natural development process of tumors and the multi-directional tumor environment. With the further development of CAR-T research, it is more important to find suitable animal models. The development of canine CAR-T will help further promote CAR-T research for human disease.

Fig.1 Common cancers that have clinical analogs in human and canine. (Mochel, et al., 2019)

One Stop Canine CAR-T Engineering Service

With years of experience in CAR-T research, Creative Biolabs provides one-stop canine CAR-T engineering services to help accelerate scientific research for global customers.

Fig.2 Overall scheme for canine CAR therapy. (Cockey & Leifer, 2023)

Comprehensive CAR Design and Construction Strategies to Assist Canine CAR-T Engineering

The classic CAR design strategies from 1^st to 5^th generation provide high specificity and effectiveness for redirecting T cells to target cancer cells. At the same time, Creative Biolabs offers a variety of special CAR designs according to clients' needs.

Fig.3 Classic CAR design strategies. (Miao, et al., 2021)

Outstanding CAR-T Gene Packaging & Delivery Solutions to Enable Canine CAR-T Engineering

Since the T cells are difficult to modify, the electroporation transfection and transposon transfection systems are more robust to complete the T-cell transfection. Creative Biolabs provides various transfection technology with strict quality control to speed up the Canine CAR-T Engineering.

Features & Benefits

Creative Biolabs is confident in providing one-stop canine CAR-T engineering services with mature experience and expertise.

Canine CAR-T Engineering Service

Published Data

Paper Title: Establishing a model system for evaluating CAR-T cell therapy using dogs with spontaneous diffuse large B cell lymphoma
Summary: The researchers designed and produced anti-canine CD20, 2^nd generation canine CAR-T cells for in vitro and in vivo functional assessment using lentiviral vectors in parallel with human CAR-T cell production. This canine CD20 CAR-T system showed promising results in the treatment of canine diffuse large B cell lymphoma (DLBCL).
Technology: Lentivectors transfection
CAR Construction Generation: 2^nd generation
Cell Type: Canine T cells
Results:

Fig.4 Design of 2^nd canine CAR-T cells. (Panjwani, et al., 2020)

Fig.5 In vitro function assessment of 2^nd canine CAR-T cells co-cultured with target cells. (Panjwani, et al., 2020)

Fig.6 In vivo function assessment of 2^nd canine CAR-T cells. (Panjwani, et al., 2020)

We offer professional guidance for canine CAR-T engineering projects, please contact us for a tailored solution for you.

References

Cockey, J.R.; Leifer, C.A. Racing CARs to veterinary immuno-oncology. Frontiers in Veterinary Science. 2023, 10:1130182.
Mochel, J.P.; et al. CAR-T cell immunotherapy in human and veterinary oncology: changing the odds against hematological malignancies. The AAPS Journal. 2019, 21:1-2.
Panjwani, M.K.; et al. Establishing a model system for evaluating CAR-T cell therapy using dogs with spontaneous diffuse large B cell lymphoma. Oncoimmunology. 2020, 9(1):1676615.
Miao, L.; et al. Obstacles and coping strategies of CAR-T cell immunotherapy in solid tumors. Frontiers in Immunology. 2021, 12:687822.