Anti-Human GD2 (h3F8) h(CD28-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF99)

Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-GD2 (h3F8) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human GD2. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-GD2 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-GD2 (h3F8) linked to 2nd generation CAR containing CD28 and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

Add to Cart

Description

Target
GD2

Components
This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
1. A CAR lentiviral vector
• Anti-GD2 (h3F8) h(CD28-CD3ζ) CAR
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. CAR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

CAR Generation
2nd-generation CAR

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-CD3ζ

Target

Gene Name
Ganglioside GD2

Full Name
Ganglioside GD2

scFv
h3F8

Host Species
Humanized

Target Species
Human

Gene ID
2583

Target Background
Gangliosides are carbohydrate-containing sphingolipids that are widely expressed in normal tissues, making most subtypes unsuitable as targets for cancer therapy. However, the disialoganglioside GD2 subtype has limited expression in normal tissues but is overexpressed across a wide range of tumors. Disialoganglioside GD2 can be considered a tumor-associated antigen and well-suited as a target for cancer therapy. Disialoganglioside GD2 is implicated in tumor development and malignant phenotypes through enhanced cell proliferation, motility, migration, adhesion, and invasion, depending on the tumor type. This provides a rationale for targeting disialoganglioside GD2 in cancer therapy with the development of anti-GD2 monoclonal antibodies and other therapeutic approaches.

Synonyms
GD2 ganglioside; GD2

Related Disease
Neuroblastoma; Melanoma

Customer Reviews and Q&As

There are currently no customer reviews or questions for Anti-Human GD2 (h3F8) h(CD28-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF99). Click the button below to contact us or submit your feedback about this product.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-Human GD2 (h3F8) h(CD28-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF99)

Description

CAR Vector

Target

Customer Reviews and Q&As

Related Products

CAR-MA Vector Products

CAR Vector Products

CAR mRNA

CAR-MA Cell Products