Anti-Human PD-L1 (Atezolizumab) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF176)

Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This kit provides a complete lentivirus packaging system to produce anti-PD-L1 (Atezolizumab) h(4-1BB-CD3ζ) CAR lentivirus, which enables CAR-T cells generation to target Human PD-L1. It contains plasmids that express all the viral proteins necessary, and CAR detection reagent, providing a full set of reagents for CAR lentivirus packaging and CAR expression evaluation. The expressed anti-PD-L1 CAR lentiviruses are HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells. These viruses constitutively express the scFv portion of anti-PD-L1 (Atezolizumab) linked to 3nd generation CAR containing CD28, 41BB and CD3ζ signaling domains. CAR detection reagent can be used to detect the positive expression rate of CAR-T cells after lentiviral transduction.

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Description

Target
PD-L1

Components
This kit contains a CAR lentiviral vector, packaging vectors, CAR detection reagent, and 293 cells.
1. A CAR lentiviral vector
• Anti-PD-L1 (Atezolizumab) h(CD28-41BB-CD3ζ) CAR
2. Three packaging vectors
• LentiArt™ pHelp1
• LentiArt™ pHelp2
• LentiArt™ pHelp3
3. CAR detection reagent
4. 293 cells

Size
10 reactions; 20 reactions; 50 reactions

Storage Conditions
For details of the storage requirements, please see the User Manual for instructions.

Applications
CAR lentivirus packaging; CAR lentivirus transfection; CAR-T cell development; CAR-T cell therapy.

CAR Vector

CAR Generation
3nd-generation CAR

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-CD28-41BB-CD3ζ

Target

Gene Name
CD274

Full Name
CD274 molecule

scFv
Atezolizumab

Host Species
Humanized

Target Species
Human

Gene ID
29126

Uniprot ID
Q9NZQ7

Target Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.

Synonyms
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Related Disease
NSCLC (Non-Small Cell Lung Cancer), Melanoma, Breast Cancer, Glioma, Lymphoma, Leukemia and Various Urinary Tract Tumors, Gastrointestinal Tumors, Germ Tract Tumors

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-Human PD-L1 (Atezolizumab) h(CD28-41BB-CD3Z) CAR Lentivirus Packaging Kit (XS-0622-YF176)

Description

CAR Vector

Target

Customer Reviews and Q&As

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