All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
Sleeping Beauty (SB) transposon, a type of nonviral integrative vectors, provides an alternative to modify primary T cells. Creative biolabs has developed SB transposon CAR vector pSBCAR1 PD-L1 (Atezolizumab) h(28ζ), which is constructed for the engineering of T cells to target human PD-L1. The T cells are genetically modified through transduction with a nonviral vector expressing scFv of anti-PD-L1 antibody linked to CD28 and CD3ζ signaling domains. And the vector product was designed for the treatment of Colorectal cancer.
CAR Construction : Atezolizumab scFv-BBζ Fig.1 Expansion of PD-L1-CAR and CD19-CAR T cells in vitro for 14 days. Data represented technical triplicates using T cells from one donor and were displayed as mean ± SEM. Liu, M., Wang, X., Li, W., Yu, X., Flores-Villanueva, P., Xu-Monette, Z. Y., ... & Li, Y. (2020). Targeting PD-L1 in non-small cell lung cancer using CAR T cells. Oncogenesis, 9(8), 1-11. |
CAR Construction : Atezolizumab scFv-BBζ Fig.2 Cytotoxic activity of PD-L1-CAR T cells after 4 and 20 h of co-culture with human NSCLC cell lines. PD-L1-CAR T cells and CD19-CAR T cells were used as effector cells at various ratios of effector (E): target (T). Liu, M., Wang, X., Li, W., Yu, X., Flores-Villanueva, P., Xu-Monette, Z. Y., ... & Li, Y. (2020). Targeting PD-L1 in non-small cell lung cancer using CAR T cells. Oncogenesis, 9(8), 1-11. |
CAR Construction : Atezolizumab scFv-BBζ Fig.3 Flow cytometry histogram of the surface antigen expression of PD-L1 in human NSCLC cell line. Data represented technical triplicates using T cells from one donor and were shown as mean ± SEM. Liu, M., Wang, X., Li, W., Yu, X., Flores-Villanueva, P., Xu-Monette, Z. Y., ... & Li, Y. (2020). Targeting PD-L1 in non-small cell lung cancer using CAR T cells. Oncogenesis, 9(8), 1-11. |
CAR Construction : Atezolizumab scFv-BBζ Fig.4 Serial bioluminescence imaging of tumor progression and regression in each group (n = 3 mice per group). Experimental design of tumor cell xenograft model treated with CAR T cells and/or irradiation. Liu, M., Wang, X., Li, W., Yu, X., Flores-Villanueva, P., Xu-Monette, Z. Y., ... & Li, Y. (2020). Targeting PD-L1 in non-small cell lung cancer using CAR T cells. Oncogenesis, 9(8), 1-11. |
CAR Construction : Atezolizumab scFv-BBζ Fig.5 Representative images of CD3 IHC in PD-L1-CAR T cell-treated and irradiation-treated NSCLC tumors. Scale bars, 100 µm. Liu, M., Wang, X., Li, W., Yu, X., Flores-Villanueva, P., Xu-Monette, Z. Y., ... & Li, Y. (2020). Targeting PD-L1 in non-small cell lung cancer using CAR T cells. Oncogenesis, 9(8), 1-11. |
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