Cytokine-Overexpressed NK Cell Engineering Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Cytokine-overexpressed natural killer (NK) cells have emerged as a promising avenue for enhancing chimeric antigen receptor NK (CAR-NK) therapy. Creative Biolabs, a pioneer in the field of cellular therapy, offers a state-of-the-art service that focuses on the engineering of cytokine-overexpressed NK cells to augment the efficacy of CAR-NK therapy.

Overview of Cytokine-Overexpressed NK Cell

The regulation of anti-tumor NK cells involves not only direct interactions between ligands and receptors but also the influence of various cytokines. Specifically, cytokines like IL-2, IL-12, IL-15, IL-18, and IL-21 play a crucial role in enhancing NK cell activation and cytotoxicity. The key roles of these highlighted cytokines can be summarized as follows:

IL-2 and IL-15 promote NK cell "priming" leading to heightened responses.
TGF-β and IL-10 contribute to the development of tolerant NK cells, which can exert beneficial effects in maintaining physiological balance.
TGF-β and IL-15 play a role in shaping regulatory NK cells.

Figure 1. Cytokines in NK cell development. (Wu, et al., 2017) Fig.1 Cytokines in NK cell development.¹

Our Service

In order to enhance the anti-tumor capabilities of CAR-NK cells, we offer a range of gene-modified L-NK cells, which include, among others, the following cytokine-induced memory-like NK cell variants:

CAR-NK Cells Overexpressing IL-2
CAR-NK Cells Overexpressing IL-12
CAR-NK Cells Overexpressing IL-15
CAR-NK Cells Overexpressing IL-18
CAR-NK Cells Overexpressing IL-21

These genetically modified NK cells are designed to bolster the immune response against tumors.

Customized Solutions

At Creative Biolabs, we understand that each patient and tumor type are unique. We work closely with our clients to determine the most suitable cytokine and delivery strategy for their CAR-NK therapy, ensuring optimal therapeutic outcomes.

Cutting-Edge Gene Editing Techniques

CRISPR-Cas9-Mediated Cytokine Overexpression
CRISPR-Cas9 technology has revolutionized the field of genetic engineering. With our expertise, we utilize this system to introduce cytokine genes into NK cells with unparalleled precision. This approach ensures that the desired cytokines are expressed at the right time and in the right place, optimizing the therapeutic impact of CAR-NK cells.
Lentiviral Vectors for Long-Term Cytokine Expression
Lentiviral vectors are another powerful tool in our arsenal. By delivering cytokine genes into NK cells using these vectors, we can achieve sustained cytokine production over an extended period. This extended cytokine release enhances the persistence and potency of CAR-NK cells, improving their ability to combat cancer.

Features of Our NK Cell Genetic Engineering Platform

Off-the-Shelf

A universal cell-based therapy that does not require personalized patient matching.

Eliminate a Step

No need to collect the patient's white blood cells. With off-the-shelf NK, this invasive procedure is eliminated.

Dosing Control

NK's clearance and multiple dosing may reduce the risk of adverse events, thereby producing a favorable safety profile.

One-Stop CAR-NK Therapy Development

Notably, as the leading cell therapeutics biotech that provides cell therapy related services, Creative Biolabs provides one-stop services for CAR-NK therapy development, including but not limited to:

If you are interested in our services, please send an email to contact us, and our team will get back to you as soon as possible.

Reference

Wu, Y., et al. (2017). Developmental and functional control of natural killer cells by cytokines. Frontiers in immunology, 8, 930.