CAR-T In Vitro Efficacy Services with OncoSolid™ Neuroendocrine Cancer Organoid Models

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Neuroendocrine Cancer Organoid Models

Neuroendocrine (NEC) neoplasm is a type of less differentiated tumor that originates from various tissues and commonly occurs in the gastroenteropancreatic tract (GEP-NEC). The incidence of this cancer is rare but presents a poor prognosis for limited clinical therapeutics and knowledge of the disease. Cancer organoids are valuable preclinical models for neuroendocrine cancer studies and potential drug and therapy development. Creative Biolabs offers a functional cancer organoids platform, including organoids of multiple cancers that retain the main histopathological and molecular features of tumors and provide CAR-T efficacy assessment in vitro.

Workflow

Fig.1 Workflow for NEC organoid establishment. (Creative Biolabs)

Workflow for NEC organoid establishment. Fig.2 Neuroendocrine organoids established from different organs. (Kawasaki, et al., 2020)

Subtypes of NEC Organoids Available

NEC cancer organoid models can be developed from various tissue of organs, including but not limited to this.

Colon-NEC organoid model
Colon-NEC organoid model
Prostate-NEC organoid model
Gastric-NEC organoid model
Esophagus-NEC organoid model
Esophagus-NEC organoid model

Colorectal-NEC organoid model
Liver-NEC organoid model
Biliary-NEC organoid model
Pancreatic-NEC organoid model
Lung-NEC organoid model
Duodenum-NEC organoid model

NEC Organoids Characterization

The original tumors established cancer organoid lines, and organoid xenografts, can be characterized to describe the correlation of the model to clinical tissues by histology, immunohistochemistry, and sequencing technology.

NEC Organoids Characterization

Application of Neuroendocrine (NE) Cancer Organoids

Organoid models have a broad application in neoplasm formation and pathology studies as well as clinical treatment discovery.

Tumor Model
Tumor Biology Research
Drug Screening
Drug Discovery
Drug Safety Test
Precision Medicine
Therapeutic Response Assessment

Key Features

The culture medium is adjusted for each tumor sample with a different origin.
Organoids stably preserve tumor features during the long-term passage.
The organoid models can be established from the pure neuroendocrine tumor and mixed neuroendocrine non-neuroendocrine neoplasms.
Support genetic modeling of organoids.

Case Report

Tittle: Patient-derived Neuroendocrine Cancer Organoid Models
Gastroenteropancreatic (GEP) neuroendocrine (NE) cancer contains neuroendocrine tumors and neuroendocrine carcinoma (NEC). 25 lines of patient-derived GEP-NE cancer organoids were established as clinical-related tumor models. GEP-NE cancer organoids retain the histological features of parental tissue and present frequent gene mutations in TP53 and RB1.
Fig.3 Histomophologically characterization of patient-derived GEP-NEC organoids and their sensitivity to drugs. (Kawasaki, et al., 2020) Picture A to C show neuroendocrine markers staining results of the clinical specimen, organoids, and organoids xenograft; Picture D to F show the drug sensitivity of colorectal-NEC is correlated to clinical cases.
Fig.4 Genetic characterization of patient-derived GEP-NEC organoids. (Kawasaki, et al., 2020) Picture A and B show mutational signatures of organoids, and picture C shows the genetic divergence of organoids.

Creative Biolabs offers high-quality organoids for tumor biology study and treatment investigation. If you'd like to know how we can be involved in your project, please contact us for detailed information.

Reference

Kawasaki, K.; et al. An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping.Cell. 2020, 183(5): 1420-1435.