All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
Sleeping Beauty (SB) transposon, a type of nonviral integrative vectors, provides an alternative to modify primary T cells. Creative biolabs has developed SB transposon CAR vector pSBCAR1 CAIX (G119) h(28BBζ), which is constructed for the engineering of T cells to target human CAIX. The T cells are genetically modified through transduction with a nonviral vector expressing scFv of anti-CAIX antibody linked to a CD28 transmembrane domain/ endodomain and CD137 (4-1BB), CD3-zeta signaling domains. And the vector product was designed for the treatment of Renal cell carcinoma.
CAR Construction : Fig.2 In vitro carbonic anhydrase activity assay on HCTl 16 membrane fragments. (A) Time course for C02 hydration with membrane-free buffer (dotted), HCT l 16-EV membrane-fragments (grey), HCTl 16-CAIX membrane fragments (black) and HCTl 16-CAIX membrane fragments blocked with MSC 8 Fab (dashed). (B) Carbonic anhydrase activity, normalized to a scale where activity measured in HCTl 16-CAIX and HCTl 16-EV membrane fragments are one and zero, respectively. Asterisks denote significant reduction in activity (MSC5, MSC 10„MSC12 and MSC8 Fab antibodies). (C) Time course for CO2 hydration with HCTl 16-EV membrane-fragments (grey), HCTl 16-CAIX membrane- fragments (black) and HCTl 16-CAIX membrane fragments blocked with MSC3 IgG (dashed black), MSC8 IgG (dotted black) or acetazolamide (dashed grey). (D) Dose-response curves for MSC 8 Fab (grey) and MSC 8 IgG (black) influence on CAIX-activity (using the same normalization algorithm as in B). |
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