Creative Biolabs offers assay services of evaluating the stability of TCR/CD3 complex to ensure the harvest of high quality TCR-modified T cells. The effective interaction of CD3 with TCR and the ξ-chain is required for the generation of activation signal in T lymphocytes. Increased stability of the TCR/CD3complex contributes to the enhanced antitumor response activity of T cells. At Creative Biolabs, the TCR/CD3 complex stability assay can be achieved via immunoprecipitation and immunoblot.
Figure: T cell receptor (TCR) complex
T-cells are a subset of lymphocytes that play a large role in the immune response targeting to various antigens. When T lymphocytes encounter antigens via the T cell antigen receptor (TCR), information about the quantity and quality of antigen engagement is relayed to the intracellular signal transduction machinery, but the TCR itself lacks a significant intracellular domain, resulting in lacking signal transduction ability. Therefore, it is usually associated with CD3 molecules which contain intracellular signaling domains to couple the TCR/CD3 complex as the downstream signaling machinery. The TCR/CD3 complex, also known as the TCR complex, is composed of six subunits which are expressed on the cell surface in a coordinate pattern after assembly in the endoplasmic reticulum (ER). The TCR/CD3 complex is assembled after a series of pairwise interactions involving the formation of dimmers of CD3 epsilon (CD3ε) with either CD3 gamma (CD3γ) or CD3 delta (CD3δ). These dimmers assemble with TCR alpha (TCR α )and TCR beta (TCRβ) chains, and finally, the CD3ξ (CD3 zeta) homodimer is added to allow export of the full complex from the ER. The main task of the TCR/CD3 complex includes two parts: ligand recognition and signal transduction from infectious agents. And it divides the tasks among its different components. In detail, the TCR contain variable regions and are responsible for antigen/MHC recognition; while the other four CD3ε, CD3γ, CD3δ and CD3ξ-chain are invariable and contain long cytoplasmic tails capable of interacting with the intracellular signal transduction machinery. Since the TCR-CD3 complex serves a critical role in the differentiation, survival, and function of T cells, and receptor triggering elicits a complex set of biological responses that serve to protect the organism from infectious agents, it is of great importance to study the TCR/CD3 complex stability of TCR-modified T cells in order to achieve an effective disease-targeting therapy.
With full understanding of the TCR/CD3 complex, Creative Biolabs is committed to offering high-standard TCR/CD3 stability assay to every client. If you have any special question about the issues of TCR products and services, please contact our scientists for help.
To discuss your demands or to request a proposal, please contact us by firstname.lastname@example.org.
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